IL1 alpha antibody [364-3B3-14]

Cat. No. GTX41494

Host	Mouse
Clonality	Monoclonal
Clone Name	364-3B3-14
Isotype	IgG1
Application	ELISA
Reactivity	Human
Package	100 μg ($399)

See all IL1 alpha products

Datasheet
Document

View product citations for antibody GTX41494 on CiteAb

APPLICATION

Application Note

ELISA: Use at a concentration of 2 - 6 μg/ml. To obtain a linear standard curve, serial dilutions of IL-1 alpha recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. ELISPOT: Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. This antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1 alpha -producing cells within mixed cell populations. Optimal dilutions/concentrations should be determined by the end user.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-1alpha

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted

Background

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.

Research Area

DATA IMAGES

GTX41494 ELISA Image

Serial dilutions of ELISA detection IL-1α recombinant protein antibody (GTX41494) ranging from 500 to 4 pg/ml were used to obtain a linear standard curve

REFERENCE

There are currently no references for IL1 alpha antibody [364-3B3-14] (GTX41494). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for IL1 alpha antibody [364-3B3-14] (GTX41494). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty

IL1 alpha antibody [364-3B3-14]

Cat. No. GTX41494

Host

Clonality

Clone Name

Isotype

Application

Reactivity