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IL1 alpha antibody

Anti-IL1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113088
Anti-IL1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113088
Anti-IL1 alpha antibody used in Western Blot (WB). GTX113088
Anti-IL1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113088

Cat. No. GTX113088

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175809

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 alpha , IL-1A , IL1 , IL1-ALPHA , IL1F1

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113088

GTX113088 IHC-P Image

IL1 alpha antibody detects IL1 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
IL1 alpha stained by IL1 alpha antibody (GTX113088) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113088

GTX113088 ICC/IF Image

IL1 alpha antibody detects IL1 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 alpha protein stained by IL1 alpha antibody (GTX113088) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-IL1 alpha antibody used in Western Blot (WB). GTX113088

GTX113088 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX113088 diluted at 1:1000

Anti-IL1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX113088

GTX113088 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IL1 alpha(GTX113088) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for IL1 alpha antibody (GTX113088). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IL1 alpha antibody (GTX113088). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169