APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human IL1B expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
Cellular Localization
Cytoplasm, cytosol,Lysosome,Secreted
Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-IL1 beta antibody [3A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60513](/templates/default/js/lightbox/images/loading.gif "Anti-IL1 beta antibody [3A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60513")
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GTX60513 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX60513 IL1 beta antibody [3A6].
Green : IL1 beta
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60513 WB Image
WB analysis of human IL1 beta (AA: 126-261) recombinant protein using GTX60513 IL1 beta antibody [3A6].
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GTX60513 WB Image
WB analysis of HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate using GTX60513 IL1 beta antibody [3A6].
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GTX60513 ELISA Image
ELISA analysis of antigen using GTX60513 IL1 beta antibody [3A6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60513 IHC-P Image
IHC-P analysis of lung cancer tissue using GTX60513 IL1 beta antibody [3A6].
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GTX60513 IHC-P Image
IHC-P analysis of human muscle tissue using GTX60513 IL1 beta antibody [3A6].
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REFERENCE
There are currently no references for IL1 beta antibody [3A6] (GTX60513). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL1 beta antibody [3A6] (GTX60513). Be the first to share your experience with this product.
![Anti-IL1 beta antibody [3A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60513](/upload/website/prouct_img/small/GTX60513/GTX60513_20170912_ICCIF_w_23061123_762.webp "Anti-IL1 beta antibody [3A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60513")
![Anti-IL1 beta antibody [3A6] used in Western Blot (WB). GTX60513](/upload/website/prouct_img/small/GTX60513/GTX60513_20170912_WB_1_w_23061123_364.webp "Anti-IL1 beta antibody [3A6] used in Western Blot (WB). GTX60513")
![Anti-IL1 beta antibody [3A6] used in Western Blot (WB). GTX60513](/upload/website/prouct_img/small/GTX60513/GTX60513_20170912_WB_w_23061123_287.webp "Anti-IL1 beta antibody [3A6] used in Western Blot (WB). GTX60513")
![Anti-IL1 beta antibody [3A6] used in ELISA (ELISA). GTX60513](/upload/website/prouct_img/small/GTX60513/GTX60513_20170912_ELISA_w_23061123_881.webp "Anti-IL1 beta antibody [3A6] used in ELISA (ELISA). GTX60513")
![Anti-IL1 beta antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX60513](/upload/website/prouct_img/small/GTX60513/GTX60513_20170912_IHC-P_w_23061123_866.webp "Anti-IL1 beta antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX60513")
![Anti-IL1 beta antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX60513](/upload/website/prouct_img/small/GTX60513/GTX60513_20170912_IHC-P_1_w_23061123_196.webp "Anti-IL1 beta antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX60513")
![Anti-IL1 beta antibody [3A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60513](/upload/website/prouct_img/normal/GTX60513/GTX60513_20170912_ICCIF_w_23061123_762.webp "Anti-IL1 beta antibody [3A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60513")
![Anti-IL1 beta antibody [3A6] used in Western Blot (WB). GTX60513](/upload/website/prouct_img/normal/GTX60513/GTX60513_20170912_WB_1_w_23061123_364.webp "Anti-IL1 beta antibody [3A6] used in Western Blot (WB). GTX60513")
![Anti-IL1 beta antibody [3A6] used in Western Blot (WB). GTX60513](/upload/website/prouct_img/normal/GTX60513/GTX60513_20170912_WB_w_23061123_287.webp "Anti-IL1 beta antibody [3A6] used in Western Blot (WB). GTX60513")
![Anti-IL1 beta antibody [3A6] used in ELISA (ELISA). GTX60513](/upload/website/prouct_img/normal/GTX60513/GTX60513_20170912_ELISA_w_23061123_881.webp "Anti-IL1 beta antibody [3A6] used in ELISA (ELISA). GTX60513")
![Anti-IL1 beta antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX60513](/upload/website/prouct_img/normal/GTX60513/GTX60513_20170912_IHC-P_w_23061123_866.webp "Anti-IL1 beta antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX60513")
![Anti-IL1 beta antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX60513](/upload/website/prouct_img/normal/GTX60513/GTX60513_20170912_IHC-P_1_w_23061123_196.webp "Anti-IL1 beta antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX60513")