APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture: GTX02972, Detection: GTX02971-02.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody is able to detect native and recombinant human IL-1β, binds to the pro-form and active form of IL-1β, and has no cross-reactivity with IL-1α.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human IL-1β
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
IL1,IL1B,IL1BETA,IL1F2,interleukin 1 beta,IL1 beta,IL-1B
Cellular Localization
Cytoplasm,Cytosol,Lysosome,Secreted,Exosome
Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-IL1 beta antibody [7P10] (Biotin) used in ELISA (ELISA). GTX02971-02](/templates/default/js/lightbox/images/loading.gif "Anti-IL1 beta antibody [7P10] (Biotin) used in ELISA (ELISA). GTX02971-02")
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GTX02971-02 ELISA Image
Sandwich ELISA analysis of human IL-1 beta protein using GTX02972 IL1 beta antibody [MT175] as coating antibody and GTX02971-02 IL1 beta antibody [7P10] (Biotin) as detecting antibody.
Substrate : pNPP
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REFERENCE
There are currently no references for IL1 beta antibody [7P10] (Biotin) (GTX02971-02). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL1 beta antibody [7P10] (Biotin) (GTX02971-02). Be the first to share your experience with this product.
![Anti-IL1 beta antibody [7P10] (Biotin) used in ELISA (ELISA). GTX02971-02](/upload/website/prouct_img/small/GTX02971-02/GTX02971-02_20210507_ELISA_w_23053123_425.webp "Anti-IL1 beta antibody [7P10] (Biotin) used in ELISA (ELISA). GTX02971-02")
![Anti-IL1 beta antibody [7P10] (Biotin) used in ELISA (ELISA). GTX02971-02](/upload/website/prouct_img/normal/GTX02971-02/GTX02971-02_20210507_ELISA_w_23053123_425.webp "Anti-IL1 beta antibody [7P10] (Biotin) used in ELISA (ELISA). GTX02971-02")