THP-1 (500 ng/mL LPS treatment for 6 hr and then 300 ng/mL Brefeldin treatment for the last 3 hr)
Predict Reactivity
Rabbit, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IL1 beta. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888424
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
Cellular Localization
Secreted
Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]
Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [GT289] (GTX634188) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634188 ICC/IF Image
IL1 beta antibody [GT289] detects IL1 beta protein by immunofluorescent analysis.Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IL1 beta stained by IL1 beta antibody [GT289] (GTX634188) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).