THP-1 (500 ng/mL LPS treatment for 6 hr and then 300 ng/mL Brefeldin treatment for the last 3 hr)
Rabbit, Rhesus Monkey(>80% identity)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IL1 beta. The exact sequence is proprietary.
Affinity purified by Protein A.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]
Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [GT289] (GTX634188) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Show More +
GTX634188 ICC/IF Image
IL1 beta antibody [GT289] detects IL1 beta protein by immunofluorescent analysis.Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IL1 beta stained by IL1 beta antibody [GT289] (GTX634188) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).