APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
1:100-1:1000 |
Assay dependent |
Note :
Sandwich ELISA
Capture: GTX636889/GTX636887, Detection: GTX636887/GTX636889
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Not tested in other applications.
Calculated MW
Product Note
The use of GTX636887 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX74034.
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
Cellular Localization
Cytoplasm, cytosol,Lysosome,Secreted
Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX636887 ELISA Image
Sandwich ELISA detection of recombinant IL1 beta using antibodies as below. Capture: IL1 beta antibody [HL1421] (GTX636887) (5 μg/mL) Detection: HRP-conjugated IL1 beta antibody [HL1423] (GTX636889) (1 μg/mL)Please notice that GTX636889 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636887. Please contact us for custom HRP-conjugated antibody.
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GTX636887 ELISA Image
Sandwich ELISA detection of recombinant IL1 beta using antibodies as below. Capture: IL1 beta antibody [HL1423] (GTX636889) (5 μg/mL) Detection: HRP-conjugated IL1 beta antibody [HL1421] (GTX636887) (1 μg/mL)Please notice that GTX636887 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636889. Please contact us for custom HRP-conjugated antibody.
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GTX636887 IHC-P Image
IL1 beta antibody [HL1421] detects secreted IL1 beta protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.IL1 beta stained by IL1 beta antibody [HL1421] (GTX636887) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636887 IHC-P Image
IL1 beta antibody [HL1421] detects secreted IL1 beta protein by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.IL1 beta stained by IL1 beta antibody [HL1421] (GTX636887) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636887 IHC-P Image
IL1 beta antibody [HL1421] detects IL1 beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.IL1 beta stained by IL1 beta antibody [HL1421] (GTX636887) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636887 ICC/IF Image
IL1 beta antibody [HL1421] detects IL1 beta protein at cytoplasm by immunofluorescent analysis.Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IL1 beta stained by IL1 beta antibody [HL1421] (GTX636887) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636887 WB Image
Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [HL1421] (GTX636887) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
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