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IL1 beta antibody [HL1423]

Recombinant antibody Orthogonal
Anti-IL1 beta antibody [HL1423] used in IHC (Paraffin sections) (IHC-P). GTX636889
Anti-IL1 beta antibody [HL1423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636889
Anti-IL1 beta antibody [HL1423] used in Western Blot (WB). GTX636889
Anti-IL1 beta antibody [HL1423] used in ELISA (ELISA). GTX636889
Anti-IL1 beta antibody [HL1423] used in ELISA (ELISA). GTX636889

Cat. No. GTX636889

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1423

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IL1 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX636889/GTX636887, Detection: GTX636887/GTX636889.
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

The use of GTX636889 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX74034.

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Cytoplasm, cytosol,Lysosome,Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody [HL1423] used in IHC (Paraffin sections) (IHC-P). GTX636889

GTX636889 IHC-P Image

IL1 beta antibody [HL1423] detects secreted IL1 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
IL1 beta stained by IL1 beta antibody [HL1423] (GTX636889) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 beta antibody [HL1423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636889

GTX636889 ICC/IF Image

Orthogonal

IL1 beta antibody [HL1423] detects IL1 beta protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody [HL1423] (GTX636889) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL1 beta antibody [HL1423] used in Western Blot (WB). GTX636889

GTX636889 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [HL1423] (GTX636889) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 beta antibody [HL1423] used in ELISA (ELISA). GTX636889

GTX636889 ELISA Image

Sandwich ELISA detection of recombinant IL1 beta using antibodies as below.

Capture: IL1 beta antibody [HL1423] (GTX636889) (5 μg/mL)
Detection: HRP-conjugated IL1 beta antibody [HL1421] (GTX636887) (1 μg/mL)
Please notice that GTX636887 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636889. Please contact us for custom HRP-conjugated antibody.

Anti-IL1 beta antibody [HL1423] used in ELISA (ELISA). GTX636889

GTX636889 ELISA Image

Sandwich ELISA detection of recombinant IL1 beta using antibodies as below.

Capture: IL1 beta antibody [HL1421] (GTX636887) (5 μg/mL)
Detection: HRP-conjugated IL1 beta antibody [HL1423] (GTX636889) (1 μg/mL)
Please notice that GTX636889 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636887. Please contact us for custom HRP-conjugated antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations