GeneTex
  • Country / Location Selection

United States (US)

IL1 beta antibody [HL1423]

Anti-IL1 beta antibody [HL1423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636889
Anti-IL1 beta antibody [HL1423] used in Western Blot (WB). GTX636889
Anti-IL1 beta antibody [HL1423] used in ELISA (ELISA). GTX636889
Anti-IL1 beta antibody [HL1423] used in ELISA (ELISA). GTX636889

Cat No. GTX636889

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1423

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX636889/GTX636887, Detection: GTX636887/GTX636889

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Cytoplasm, cytosol,Lysosome,Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody [HL1423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636889

GTX636889 ICC/IF Image

IL1 beta antibody [HL1423] detects IL1 beta protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody [HL1423] (GTX636889) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL1 beta antibody [HL1423] used in Western Blot (WB). GTX636889

GTX636889 WB Image

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [HL1423] (GTX636889) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 beta antibody [HL1423] used in ELISA (ELISA). GTX636889

GTX636889 ELISA Image

Sandwich ELISA detection of recombinant IL1 beta using antibodies as below.

Capture: IL1 beta antibody [HL1423] (GTX636889) (5 μg/mL)
Detection: HRP-conjugated IL1 beta antibody [HL1421] (GTX636887) (1 μg/mL)
Please notice that GTX636887 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636889. Please contact us for custom HRP-conjugated antibody.

Anti-IL1 beta antibody [HL1423] used in ELISA (ELISA). GTX636889

GTX636889 ELISA Image

Sandwich ELISA detection of recombinant IL1 beta using antibodies as below.

Capture: IL1 beta antibody [HL1421] (GTX636887) (5 μg/mL)
Detection: HRP-conjugated IL1 beta antibody [HL1423] (GTX636889) (1 μg/mL)
Please notice that GTX636889 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636887. Please contact us for custom HRP-conjugated antibody.

REFERENCE

There are currently no references for IL1 beta antibody [HL1423] (GTX636889). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IL1 beta antibody [HL1423] (GTX636889). Be the first to share your experience with this product.
Package List Price ($)
$ 379
$ 169
Custom Conjugations