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IL1 beta antibody, Internal

Anti-IL1 beta antibody, Internal used in Western Blot (WB). GTX81192
Anti-IL1 beta antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81192
Anti-IL1 beta antibody, Internal used in Western Blot (WB). GTX81192
Anti-IL1 beta antibody, Internal used in Flow cytometry (FACS). GTX81192

Cat. No. GTX81192

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human, Mouse
Package
400 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:25
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Predict Reactivity

Rabbit, Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 148-174 amino acids of human IL1B.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Cytoplasm, cytosol,Lysosome,Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody, Internal used in Western Blot (WB). GTX81192

GTX81192 WB Image

WB analysis of NCI-H460 cell lysate (35ug/lane) using GTX81192 IL1 beta antibody, Internal.

Anti-IL1 beta antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81192

GTX81192 IHC-P Image

IHC-P analysis of human lung carcinoma using GTX81192 IL1 beta antibody, Internal.

Anti-IL1 beta antibody, Internal used in Western Blot (WB). GTX81192

GTX81192 WB Image

WB analysis of various samples using GTX81192 IL1 beta antibody, Internal.
Lane 1: human spleen lysate
Lane 2: mouse brain lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-IL1 beta antibody, Internal used in Flow cytometry (FACS). GTX81192

GTX81192 FACS Image

FACS analysis of MCF-7 cells using GTX81192 IL1 beta antibody, Internal.
Green : IL1 beta
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399