APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:10-1:50 |
| 1:25 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 148-174 amino acids of human IL1B.
Purification
Protein A purified, followed by peptide affinity purification.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
Cellular Localization
Cytoplasm, cytosol,Lysosome,Secreted
Background
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX81192 FACS Image
FACS analysis of MCF-7 cells using GTX81192 IL1 beta antibody, Internal.
Green : IL1 beta
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25
|
|
|
GTX81192 WB Image
WB analysis of NCI-H460 cell lysate (35ug/lane) using GTX81192 IL1 beta antibody, Internal.
|
|
|
GTX81192 WB Image
WB analysis of various samples using GTX81192 IL1 beta antibody, Internal.
Lane 1: human spleen lysate
Lane 2: mouse brain lysate
Loading : 20 μg per lane
Dilution : 1:2000
|
|
|
GTX81192 IHC-P Image
IHC-P analysis of human lung carcinoma using GTX81192 IL1 beta antibody, Internal.
|
REFERENCE
There are currently no references for IL1 beta antibody, Internal (GTX81192). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL1 beta antibody, Internal (GTX81192). Be the first to share your experience with this product.
