GeneTex
United States (US)

IL1 beta antibody

Orthogonal
GTX100793_39959_20151123_IHC-P_19011506_448.jpg
GTX100793_39959_20161222_WB_LPS_ATP_19011506_282.jpg
GTX100793_39959_20180919_ICC_IF_19011519_154.jpg

Cat No. GTX100793

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

THP-1(500 ng/ml LPS treatment for 4 hr and 1 mM ATP treatment for 1.5 hr) , THP-1 conditioned medium(500 ng/ml LPS treatment for 4 hr and 1 mM ATP treatment for 1.5 hr)

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 1 Beta,Il-1,Il1-Beta,Il1F2,Il1B

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100793_39959_20151123_IHC-P_19011506_448.jpg

GTX100793 IHC-P Image

IL1 beta antibody [N1C3] detects IL1B protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100793_39959_20161222_WB_LPS_ATP_19011506_282.jpg

GTX100793 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500. (WCE: whole cell extract; CM: conditioned medium)

GTX100793_39959_20180919_ICC_IF_19011519_154.jpg

GTX100793 ICC/IF Image

IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody (GTX100793) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
Glycerol.pdf
Thimerosal.pdf