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IL1 beta antibody

Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100793
Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX100793
Anti-IL1 beta antibody used in Western Blot (WB). GTX100793

Cat No. GTX100793

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

THP-1 conditioned medium(500 ng/ml LPS treatment for 4 hr and 1 mM ATP treatment for 1.5 hr)

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037241

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100793

GTX100793 ICC/IF Image

IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody (GTX100793) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (phalloidin) diluted at 1:100.

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX100793

GTX100793 IHC-P Image

IL1 beta antibody [N1C3] detects IL1B protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 beta antibody used in Western Blot (WB). GTX100793

GTX100793 WB Image

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500. (WCE: whole cell extract; CM: conditioned medium)

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169