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IL1 beta antibody

Orthogonal
Anti-IL1 beta antibody used in Western Blot (WB). GTX130021
Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130021

Cat. No. GTX130021

Reference 8
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IL1 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Predict Reactivity

Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.20 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL1 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886157

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody used in Western Blot (WB). GTX130021

GTX130021 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX130021) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130021

GTX130021 ICC/IF Image

Orthogonal

IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody (GTX130021) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Jeong GU et al. J Virol 2023; 97 (6) : e0055623 Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.
X Zhang et al. Proc Natl Acad Sci U S A 2023; 120 (27) : e2300204120 Inhibition of AIM2 inflammasome activation by SOX/ORF37 promotes lytic replication of Kaposi's sarcoma-associated herpesvirus
Application : WB
Lin CY et al. Sci Adv 2023; 9 (14) : eade9944 Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response.
Application : WB
Reactivity : Human
Wei KC et al. Microbiol Spectr 2023; 11 (1) : e0358622 Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1.
Law YY et al. Aging (Albany NY) 2021; 13 (13) : 17227-17236 miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts.
Application : WB
Reactivity : Human
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REVIEW

IL1 beta antibody Cat. No. GTX130021

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-IL1 beta antibody used in Western Blot (WB). GTX130021
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MH7A cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MH7A whole cell lysate, Lane 2: treated (pm2.5; 3ug/mL) MH7A whole cell lysate, Lane 3:treated (pm2.5; 10ug/mL) MH7A whole cell lysate
Anti-IL1 beta antibody used in Western Blot (WB). GTX130021
Date : Anonymous submitted on 01-Aug-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 cells
Amount used : 30 μg
Blocking : 5% skim milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:4000, 25°C, 12Hr
Lane Description : LPS (1μg/ml, 2 hr) and Nigericin (5μM, 1.5 hr) treated THP-1 cell condition medium.
Submit a Review
Application Reference
Jeong GU et al. J Virol 2023; 97 (6):e0055623 Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.
X Zhang et al. Proc Natl Acad Sci U S A 2023; 120 (27):e2300204120 Inhibition of AIM2 inflammasome activation by SOX/ORF37 promotes lytic replication of Kaposi's sarcoma-associated herpesvirus
Application : WB
Lin CY et al. Sci Adv 2023; 9 (14):eade9944 Small-molecule PIK-93 modulates the tumor microenvironment to improve immune checkpoint blockade response.
Application : WB
Reactivity : Human
Wei KC et al. Microbiol Spectr 2023; 11 (1):e0358622 Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1.
Law YY et al. Aging (Albany NY) 2021; 13 (13):17227-17236 miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts.
Application : WB
Reactivity : Human
Tsai MH et al. Cells 2020; 9 (8) Ischemic Stroke Risk Associated with Mitochondrial Haplogroup F in the Asian Population.
Application : WB
Reactivity : Human
Nam SM et al. Nutrients 2019; 11 (1) Ascorbic Acid Mitigates D-galactose-Induced Brain Aging by Increasing Hippocampal Neurogenesis and Improving Memory Function.
Application : WB
Tokuju Okano et al. European Journal of Immunology 2018; (Epub) Porphyromonas gingivalis triggers NLRP3-mediated inflammasome activation in macrophages in a bacterial gingipains-independent manner.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169