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IL1 beta antibody

Orthogonal
Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55675
Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX55675
Anti-IL1 beta antibody used in Western Blot (WB). GTX55675
Anti-IL1 beta antibody used in Western Blot (WB). GTX55675

Cat. No. GTX55675

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μl ($399)
See all IL1 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human IL1B (NP_000567.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887933

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Cytoplasm, cytosol,Lysosome,Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55675

GTX55675 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX55675 IL1 beta antibody.
Blue : DAPI
Dilution : 1:100

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX55675

GTX55675 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX55675 IL1 beta antibody.
Blue : DAPI
Dilution : 1:100

Anti-IL1 beta antibody used in Western Blot (WB). GTX55675

GTX55675 WB Image

WB analysis of mouse liver tissue lysate using GTX55675 IL1 beta antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IL1 beta antibody used in Western Blot (WB). GTX55675

GTX55675 WB Image

Orthogonal

The data was published in the 2022 in Vet Res. PMID: 35123552

REFERENCE

Application Reference

Yu Fu et al. Evid Based Complement Alternat Med 2022; 2022 : 9131178 Biejiajian Pill Ameliorates Diabetes-Associated Atherosclerosis through Inhibition of the NLRP3 Inflammasome.
Application : WB
Reactivity : Mouse
Wang X et al. Vet Res 2022; 53 (1) : 10 Staphylococcus aureus mediates pyroptosis in bovine mammary epithelial cell via activation of NLRP3 inflammasome.
Application : WB
Chen CM et al. Stem Cell Res Ther 2017; 8 (1) : 180 Surfactant effects on the viability and function of human mesenchymal stem cells: in vitro and in vivo assessment.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399