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IL1 beta antibody

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX55675
Anti-IL1 beta antibody used in Western Blot (WB). GTX55675
Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55675

Cat. No. GTX55675

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human IL1B (NP_000567.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887933

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Cytoplasm, cytosol,Lysosome,Secreted

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX55675

GTX55675 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX55675 IL1 beta antibody.
Blue : DAPI
Dilution : 1:100

Anti-IL1 beta antibody used in Western Blot (WB). GTX55675

GTX55675 WB Image

WB analysis of mouse liver tissue lysate using GTX55675 IL1 beta antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55675

GTX55675 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX55675 IL1 beta antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399