IL1 beta antibody

Name: IL1 beta antibody
Brand: GeneTex
SKU: GTX74034
Price: 469.00 USD
Rating: 5 (1 reviews)

Cat. No. GTX74034

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Cat. No. GTX74034

GTX74034 IHC-P Image

IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.
Red: NF-H, a neuron marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX74034 WB Image

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX74034 WB Image

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX74034 WB Image

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GTX74034 ELISA Image

An ELISA plate is coated with 50 μL of IL1 beta recombinant protein at concentration of 5 μg/mL. The coated protein is detected with anti-IL1 beta antibody (GTX74034) at concentration ranged from 0.01 to 0.15 μg/mL.

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GTX74034 IHC-Fr Image

IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

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GTX74034 ICC/IF Image

IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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GTX74034 WB Image

The data was published in the journal Nat Commun in 2015.PMID: 26104484

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GTX74034 WB Image

The data was published in the 2019 in Commun Biol. PMID: 31602405

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GTX74034 WB Image

The data was published in the 2022 in Sci Rep. PMID: 35256715

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GTX74034 WB Image

The data was published in the 2022 in Sci Rep. PMID: 35256715

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GTX74034 WB Image

The data was published in the 2022 in Sci Rep. PMID: 35256715

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This IL1 beta antibody (GTX74034) is a rabbit polyclonal antibody validated for detection of IL1 beta by WB, ICC/IF, IHC-P, IHC-Fr, and ELISA. Reactivity has been shown in Human, Mouse, and Rat. This product is cited in 130 published studies.

Host

Rabbit
Clonality

Polyclonal
Isotype

IgG
Applications

WB ICC/IF IHC-P IHC-Fr ELISA
Reactivity

Human, Mouse, Rat

Summary

Interleukin-1 beta (IL-1 beta) antibody detects IL-1 beta protein, which is a proinflammatory cytokine produced by active macrophages. In recent reports, IL-1 beta activation via caspase-1 can be induced by infectious pathogens in a process known as “pyroptosis”, a form of programmed cell death that initiates an inflammatory antimicrobial response. IL-1 beta is also a key factor in cytokine storms associated with both infectious and non-infectious diseases.