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IL1 beta antibody

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX74034
Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX74034
Anti-IL1 beta antibody used in ELISA (ELISA). GTX74034
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Anti-IL1 beta antibody used in IHC (Frozen sections) (IHC-Fr). GTX74034
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034

Cat No. GTX74034

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Functional Assay, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($469)
PRODUCT

Summary

Interleukin-1 beta (IL-1 beta) antibody detects IL-1 beta protein, which is a proinflammatory cytokine produced by active macrophages. In recent reports, IL-1 beta activation via caspase-1 can be induced by infectious pathogens in a process known as “pyroptosis”, a form of programmed cell death that initiates an inflammatory antimicrobial response. IL-1 beta is also a key factor in cytokine storms associated with both infectious and non-infectious diseases.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
ELISA 0.5-2μg/ml
Functional Assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Raw264.7 (50 ng/ml LPS treatment for 4 hr) , *skin tissue

Specificity/Sensitivity

This antibody may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with GTX74033 as the detection reagent.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant murine IL-1 beta

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX74034

GTX74034 IHC-P Image

IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.
Red: NF-H, a neuron marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX74034

GTX74034 ICC/IF Image

IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL1 beta antibody used in ELISA (ELISA). GTX74034

GTX74034 ELISA Image

An ELISA plate is coated with 50 μL of IL1 beta recombinant protein at concentration of 5 μg/mL. The coated protein is detected with anti-IL1 beta antibody (GTX74034) at concentration ranged from 0.01 to 0.15 μg/mL.

Anti-IL1 beta antibody used in Western Blot (WB). GTX74034

GTX74034 WB Image

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 beta antibody used in IHC (Frozen sections) (IHC-Fr). GTX74034

GTX74034 IHC-Fr Image

IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL1 beta antibody used in Western Blot (WB). GTX74034

GTX74034 WB Image

Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 beta antibody used in Western Blot (WB). GTX74034

GTX74034 WB Image

Untreated (–) and treated (+) THP-1 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IL1 beta antibody used in Western Blot (WB). GTX74034

GTX74034 WB Image

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX132080) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

IL1 beta antibody Cat No. GTX74034

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Mar-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : skin tissue
Amount used : 30μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 469