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IL1 beta antibody

Orthogonal
Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX74034
Anti-IL1 beta antibody used in IHC (Frozen sections) (IHC-Fr). GTX74034
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Anti-IL1 beta antibody used in ELISA (ELISA). GTX74034
Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX74034
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034

Cat. No. GTX74034

Reference 69
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($469)
See all IL1 beta products
PRODUCT

Summary

Interleukin-1 beta (IL-1 beta) antibody detects IL-1 beta protein, which is a proinflammatory cytokine produced by active macrophages. In recent reports, IL-1 beta activation via caspase-1 can be induced by infectious pathogens in a process known as “pyroptosis”, a form of programmed cell death that initiates an inflammatory antimicrobial response. IL-1 beta is also a key factor in cytokine storms associated with both infectious and non-infectious diseases.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
ELISA 0.5-2μg/ml
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Raw264.7 (500ng/ml LPS treatment for 6 hr)

Predict Reactivity

Mouse (100%)| Rat (90%)| Rabbit (81%)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant murine IL1 beta

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_378141

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 beta , IL-1beta , Il-1b

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX74034

GTX74034 IHC-P Image

IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.
Red: NF-H, a neuron marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL1 beta antibody used in IHC (Frozen sections) (IHC-Fr). GTX74034

GTX74034 IHC-Fr Image

IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL1 beta antibody used in Western Blot (WB). GTX74034

GTX74034 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IL1 beta antibody used in Western Blot (WB). GTX74034

GTX74034 WB Image

Orthogonal

Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1 beta antibody used in ELISA (ELISA). GTX74034

GTX74034 ELISA Image

An ELISA plate is coated with 50 μL of IL1 beta recombinant protein at concentration of 5 μg/mL. The coated protein is detected with anti-IL1 beta antibody (GTX74034) at concentration ranged from 0.01 to 0.15 μg/mL.

Anti-IL1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX74034

GTX74034 ICC/IF Image

IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL1 beta antibody used in Western Blot (WB). GTX74034

GTX74034 WB Image

Orthogonal

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX132080) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Mostafa M Eltobgy et al. Proc Natl Acad Sci U S A 2022; 119 (21) : e2202012119 Caspase-4/11 exacerbates disease severity in SARS-CoV-2 infection by promoting inflammation and immunothrombosis.
Application : IHC-Fr
Reactivity : Mouse
Rui Ding et al. J Neuroinflammation 2022; 19 (1) : 137 Activating cGAS-STING axis contributes to neuroinflammation in CVST mouse model and induces inflammasome activation and microglia pyroptosis.
Application : WB, IHC-P
Reactivity : Mouse
Lee SH et al. Sci Rep 2022; 12 (1) : 3681 Povidone iodine suppresses LPS-induced inflammation by inhibiting TLR4/MyD88 formation in airway epithelial cells.
Application : WB
Reactivity : Human
Seungjin Ryu et al. Elife 2021; 10 Ketogenic diet restrains aging-induced exacerbation of coronavirus infection in mice.
Application : WB
Reactivity : Mouse
Yan Zhang et al. Blood Adv 2021; 5 (12) : 2619-2623 Inflammasome activation promotes venous thrombosis through pyroptosis.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

IL1 beta antibody Cat. No. GTX74034

Rating
( Average 5 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 5 users reviews)
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Sci Rep. PMID: 35256715
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the 2022 in Sci Rep. PMID: 35256715
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Sci Rep. PMID: 35256715
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2015.PMID: 26104484
Anti-IL1 beta antibody used in Western Blot (WB). GTX74034
Date : Anonymous submitted on 13-Mar-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : skin tissue
Amount used : 30μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Mostafa M Eltobgy et al. Proc Natl Acad Sci U S A 2022; 119 (21):e2202012119 Caspase-4/11 exacerbates disease severity in SARS-CoV-2 infection by promoting inflammation and immunothrombosis.
Application : IHC-Fr
Reactivity : Mouse
Rui Ding et al. J Neuroinflammation 2022; 19 (1):137 Activating cGAS-STING axis contributes to neuroinflammation in CVST mouse model and induces inflammasome activation and microglia pyroptosis.
Application : WB, IHC-P
Reactivity : Mouse
Lee SH et al. Sci Rep 2022; 12 (1):3681 Povidone iodine suppresses LPS-induced inflammation by inhibiting TLR4/MyD88 formation in airway epithelial cells.
Application : WB
Reactivity : Human
Xinbo Zhang et al. Proc Natl Acad Sci U S A 2021; 118 (47) Desmosterol suppresses macrophage inflammasome activation and protects against vascular inflammation and atherosclerosis.
Application : WB
Reactivity : Mouse
Oc?ane Dufies et al. Nat Microbiol 2021; 6 (3):401-412 Escherichia coli Rho GTPase-activating toxin CNF1 mediates NLRP3 inflammasome activation via p21-activated kinases-1/2 during bacteraemia in mice.
Application : WB
Reactivity : Human
Szu-Han Chen et al. Int J Mol Sci 2021; 22 (9) Investigation of Neuropathology after Nerve Release in Chronic Constriction Injury of Rat Sciatic Nerve.
Application : IHC-P
Reactivity : Rat
Seungjin Ryu et al. Elife 2021; 10 Ketogenic diet restrains aging-induced exacerbation of coronavirus infection in mice.
Application : WB
Reactivity : Mouse
Yan Zhang et al. Blood Adv 2021; 5 (12):2619-2623 Inflammasome activation promotes venous thrombosis through pyroptosis.
Application : WB
Reactivity : Mouse
Lee KT et al. Aging (Albany NY) 2021; 13 (18):22490-22501 Nesfatin-1 facilitates IL-1β production in osteoarthritis synovial fibroblasts by suppressing miR-204-5p synthesis through the AP-1 and NF-κB pathways.
Application : WB
Reactivity : Human
Li H et al. J Inflamm Res 2021; 14:4839-4858 Neutrophil Extracellular Traps Augmented Alveolar Macrophage Pyroptosis via AIM2 Inflammasome Activation in LPS-Induced ALI/ARDS.
Application : WB
Su C.H. et al. Journal of Functional Foods 2021; 86 Betulin suppresses TNF-α and IL-1β production in osteoarthritis synovial fibroblasts by inhibiting the MEK/ERK/NF-κB pathway.
Application : WB, IHC-P
Reactivity : Human
Mayes-Hopfinger L et al. Nat Commun 2021; 12 (1):4546 Chloride sensing by WNK1 regulates NLRP3 inflammasome activation and pyroptosis.
Reactivity : Mouse
Jamieson S et al. Sci Rep 2021; 11 (1):10531 Inflammatory responses to metal oxide ceramic nanopowders.
Application : WB
Reactivity : Human
Guan C et al. Theranostics 2021; 11 (8):3981-3995 SIRT3-mediated deacetylation of NLRC4 promotes inflammasome activation.
Application : WB
Reactivity : Mouse
Xingyan Li et al. Nat Commun 2020; 11 (1):6364 Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners.
Application : WB
Reactivity : Human
Liu J et al. Front Pharmacol 2020; 11:812 Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production.
Hou B et al. Cell Death Dis 2020; 11 (5):377 Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype.
Fuster JJ et al. Cell Rep 2020; 33 (4):108326 TET2-Loss-of-Function-Driven Clonal Hematopoiesis Exacerbates Experimental Insulin Resistance in Aging and Obesity.
Application : WB
Reactivity : Mouse
Fann DY et al. Neuromolecular Med 2020; 22 (4):474-483 CD137 Ligand-CD137 Interaction is Required For Inflammasome-Associated Brain Injury Following Ischemic Stroke.
Application : WB
Reactivity : Mouse
Zhou Y et al. Oncol Lett 2020; 20 (6):298 Anti-CD206 antibody-conjugated Fe3O4-based PLGA nanoparticles selectively promote tumor-associated macrophages to polarize to the pro-inflammatory subtype.
Application : WB
Reactivity : Mouse
Shi Y et al. J Cell Mol Med 2020; 24 (19):11397-11408 Circular RNA VMA21 ameliorates sepsis-associated acute kidney injury by regulating miR-9-3p/SMG1/inflammation axis and oxidative stress.
Application : WB
Reactivity : Rat
Chang JW et al. Cereb Cortex 2020; 30 (8):4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
Application : WB
Reactivity : Rat
Donado CA et al. Cell Rep 2020; 31 (1):107466 A Two-Cell Model for IL-1β Release Mediated by Death-Receptor Signaling.
Application : WB
Reactivity : Mouse
Martins F et al. Cancers (Basel) 2020; 12 (4) Hypoxia and Macrophages Act in Concert Towards a Beneficial Outcome in Colon Cancer.
Application : WB
Reactivity : Human
Govindappa PK et al. Int Immunopharmacol 2020; 82:106330 An effective erythropoietin dose regimen protects against severe nerve injury-induced pathophysiological changes with improved neural gene expression and enhances functional recovery.
Application : IHC-P
Reactivity : Mouse
Qinyang Wang et al. Nature 2020; (Epub) A bioorthogonal system reveals antitumour immune function of pyroptosis.
Application : WB
Reactivity : Mouse
Wu C et al. Immunity 2019; 50 (6):1401-1411.e4 Inflammasome Activation Triggers Blood Clotting and Host Death through Pyroptosis.
Ising C et al. Nature 2019; 575 (7784):669-673 NLRP3 inflammasome activation drives tau pathology.
Application : WB
Reactivity : Human, Mouse
de Vasconcelos NM et al. Life Sci Alliance 2019; 2 (1) DPP8/DPP9 inhibition elicits canonical Nlrp1b inflammasome hallmarks in murine macrophages.
Mamantopoulos M et al. Front Immunol 2019; 10:2463 El Tor Biotype Vibrio cholerae Activates the Caspase-11-Independent Canonical Nlrp3 and Pyrin Inflammasomes.
Application : WB
Reactivity : Mouse
Vande Walle L et al. PLoS Biol 2019; 17 (9):e3000354 MCC950/CRID3 potently targets the NACHT domain of wild-type NLRP3 but not disease-associated mutants for inflammasome inhibition.
Application : WB
Reactivity : Human
Wei L et al. Brain Res 2019;:146423 Complement C3 participates in the function and mechanism of traumatic brain injury at simulated high altitude.
Application : IHC-P
Reactivity : Rat
Reitz CJ et al. Commun Biol 2019; 2:353 SR9009 administered for one day after myocardial ischemia-reperfusion prevents heart failure in mice by targeting the cardiac inflammasome.
Application : WB
Reactivity : Mouse
Lieselotte Vande Walle et al. bioRxiv 2019; (Epub) MCC950/CRID3 potently targets the NACHT domain of wildtype NLRP3 but not disease-associated mutants for inflammasome inhibition.
Application : WB
Reactivity : Mouse
Han CJ et al. Acta Pharmacol Sin 2019; (Epub) The oncometabolite 2-hydroxyglutarate inhibits microglial activation via the AMPK/mTOR/NF-κB pathway.
Application : WB
Reactivity : Mouse
Dubois H et al. PLoS Pathog 2019; 15 (4):e1007709 Nlrp3 inflammasome activation and Gasdermin D-driven pyroptosis are immunopathogenic upon gastrointestinal norovirus infection.
Application : WB
Reactivity : Mouse
Ji Y et al. Front Pharmacol 2019; 10:142 Vildagliptin Reduces Stenosis of Injured Carotid Artery in Diabetic Mouse Through Inhibiting Vascular Smooth Muscle Cell Proliferation via ER Stress/NF-κB Pathway.
Application : WB
Reactivity : Mouse
D'Eliseo D et al. J Ethnopharmacol 2019; 233:41-46 In vitro studies on anti-inflammatory activities of kiwifruit peel extract in human THP-1 monocytes.
Application : WB
Reactivity : Human
Kanneganti A et al. J Exp Med 2018; 215 (6):1519-1529 GSDMD is critical for autoinflammatory pathology in a mouse model of Familial Mediterranean Fever.
Ravussin A et al. Cell Rep 2018; 24 (5):1085-1092.e6 Loss of Nucleobindin-2 Causes Insulin Resistance in Obesity without Impacting Satiety or Adiposity.
Hafner-Bratkovi? I et al. Nat Commun 2018; 9 (1):5182 NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway.
Application : WB
Reactivity : Mouse
Skinner SC et al. Diabetes Care 2018; 41 (12):2595-2602 Increased Prevalence of Type 2 Diabetes-Related Complications in Combined Type 2 Diabetes and Sickle Cell Trait.
Application : ELISA
Reactivity : Human
Pei-Ying Li et al. RSC Adv. 2018; 8:31515-31528 Alpinumisoflavone attenuates lipopolysaccharide-induced acute lung injury by regulating the effects of anti-oxidation and anti-inflammation both in vitro and.
Application : WB
Reactivity : Mouse
Zhou Y et al. Cancer Med 2018; (Epub) Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl-p53 pathway.
Application : WB
Reactivity : Mouse
Singh AK et al. Sci Rep 2018; 8 (1):5932 Novel Indole-fused benzo-oxazepines (IFBOs) inhibit invasion of hepatocellular carcinoma by targeting IL-6 mediated JAK2/STAT3 oncogenic signals.
Lee BL et al. Sci Rep 2018; 8 (1):3788 ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Reactivity : Mouse
Van Opdenbosch N et al. Cell Rep 2017; 21 (12):3427-3444 Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC4.
Application : WB
Reactivity : Mouse
Evavold CL et al. Immunity 2017; (Epub) The Pore-Forming Protein Gasdermin D Regulates Interleukin-1 Secretion from Living Macrophages.
Application : WB
Yang X et al. Cell Res 2017; (Epub) Structural basis for specific flagellin recognition by the NLR protein NAIP5.
Application : WB
Reactivity : Human
Mishra SK et al. Autophagy 2017;:1-46 CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation.
Kader M et al. PLoS Pathog 2017; 13 (10):e1006644 MyD88-dependent inflammasome activation and autophagy inhibition contributes to Ehrlichia-induced liver injury and toxic shock.
Application : WB
Reactivity : Mouse
Zanoni I et al. Immunity 2017; 47 (4):697-709.e3 By Capturing Inflammatory Lipids Released from Dying Cells, the Receptor CD14 Induces Inflammasome-Dependent Phagocyte Hyperactivation.
Application : WB
Reactivity : Mouse
Chen YC et al. J Formos Med Assoc 2017; Anti-inflammation performance of curcumin-loaded mesoporous calcium silicate cement.
Application : WB
Reactivity : Human
Kuo CY et al. Cancer Res 2017; (Epub) VHL inactivation in precancerous kidney cells induces an inflammatory response via ER stress-activated IRE1α signaling.
Application : WB
Reactivity : Human
Fuster JJ et al. Science 2017; 355 (6327):842-847 Clonal hematopoiesis associated with TET2 deficiency accelerates atherosclerosis development in mice.
Application : WB
Reactivity : Mouse
Hassane M et al. Mucosal Immunol 2017; (Epub) Neutrophilic NLRP3 inflammasome-dependent IL-1β secretion regulates the γδT17 cell response in respiratory bacterial infections.
Application : WB
Reactivity : Mouse
Spadaro O et al. Cell Rep 2016; 14 (7):1571-1580 Growth Hormone Receptor Deficiency Protects against Age-Related NLRP3 Inflammasome Activation and Immune Senescence.
Van Gorp H et al. Proc Natl Acad Sci U S A 2016; 113 (50):14384-14389 Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation.
Application : WB
Reactivity : Mouse
Zanoni I et al. Science 2016; 352 (6290):1232-6 An endogenous caspase-11 ligand elicits interleukin-1 release from living dendritic cells.
Application : ELISA
Reactivity : Mouse
Qu Y et al. J Exp Med 2016; (Epub) NLRP3 recruitment by NLRC4 during Salmonella infection.
Application : WB
Reactivity : Mouse
Camell CD et al. J Biol Chem 2015; 290 (49):29402-13 macrophage-specific de novo synthesis of ceramide is dispensable for inflammasome-driven inflammation and * resistance in obesity.
Application : WB
Reactivity : Mouse
Patankar YR et al. Am J Physiol Lung Cell Mol Physiol 2015; 309 (8):L902-13 Differential ASC requirements reveal a key role for neutrophils and a noncanonical IL-1β response to Pseudomonas aeruginosa.
Kang S et al. Nat Commun 2015; 6 (Epub):7515 Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3.
Application : WB
Reactivity : Mouse
Youm YH et al. Nat Med 2015; 21 (3):263-9 The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease.
Application : WB
Reactivity : Mouse
Van Opdenbosch N et al. Nat Commun 2014; 5:3209 Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation.
Torres IM et al. Infect Immun 2014; Acidosis Potentiates the Host Pro-Inflammatory IL-1β Response to Pseudomonas aeruginosa Infection.
Application : WB
Reactivity : Mouse
Walle LV et al. Nature 2014; 512 (7512):69-73 Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis
Application : WB
Reactivity : Mouse
Sham HP et al. PLoS Pathog 2013; 9 (8):e1003539 SIGIRR, a negative regulator of TLR/IL-1R signalling promotes Microbiota dependent resistance to colonization by enteric bacterial pathogens.
Application : WB
Reactivity : Mouse
Kayagaki N et al. Nature 2011; 479 (7371):117-21 Non-canonical inflammasome activation targets caspase-11.
Application : WB
Reactivity : Mouse
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Package List Price ($)
$ 469
Currently in production