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IL10 antibody [12G8] (Biotin)

Anti-IL10 antibody [12G8] (Biotin) used in ELISA (ELISA). GTX15764

Cat. No. GTX15764

Host

 Rat

Clonality

 Monoclonal

Clone Name

 12G8

Isotype

 IgG2a

Application

 WB, FACS, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse
Package
250 μg ($339)
See all IL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX15625, Detection : GTX15764
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 4% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-10 (COS cell-derived)

Purification

Protein G purified

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-IL10 antibody [12G8] (Biotin) used in ELISA (ELISA). GTX15764

GTX15764 ELISA Image

Sandwich ELISA analysis of serial diluted human IL-10 recombinant protein(50ul per well) using IL10 antibody
Coating : GTX15625 IL10 antibody [9D7] (2 μg/mL)
Detection : GTX15764 IL10 antibody [12G8] (Biotin) (0.3 μg/mL)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 339