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IL10 antibody [51E6] (Biotin)

Anti-IL10 antibody [51E6] (Biotin) used in ELISA (ELISA). GTX02949-02

Cat. No. GTX02949-02

Host

 Rat

Clonality

 Monoclonal

Clone Name

 51E6

Isotype

 IgG2b

Application

 ELISA, Sandwich ELISA

Reactivity

 Mouse
Package
250 μg ($399)
See all IL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX02951, Detection: GTX02949-02.
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Product Note

This antibody is able to detect native and recombinant mouse IL-10.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse IL-10

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted

Background

This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-IL10 antibody [51E6] (Biotin) used in ELISA (ELISA). GTX02949-02

GTX02949-02 ELISA Image

Sandwich ELISA analysis of mouse IL-10 protein using GTX02951 IL10 antibody [MT60] as coating antibody and GTX02949-02 IL10 antibody [51E6] (Biotin) as detecting antibody.
Substrate : pNPP

REFERENCE

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REVIEW

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Package List Price ($)
$ 399