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IL10 antibody [GT5111]

Orthogonal
Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359
Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359
Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359

Cat. No. GTX632359

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT5111

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

3x Flag human IL-10-transfected 293T

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Guinea pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL10. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888308

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359

GTX632359 IHC-P Image

IL10 antibody [GT5111] detects IL10 protein in mouse thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
IL10 antibody [GT5111] (GTX632359) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359

GTX632359 WB Image

IL10 antibody [GT5111] detects IL10 protein by western blot analysis. Non-transfected (-) and IL-10-transfected (+, including 3x Flag-tag) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody [GT5111] (GTX632359) at a dilution of 1:5000.

Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359

GTX632359 IHC-P Image

IL10 antibody [GT5111] detects IL10 protein in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
IL10 antibody [GT5111] (GTX632359) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Mang Y et al. Ann Transl Med 2022; 10 (19) : 1066 Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
Application : WB
Reactivity : Rat
Fujita Y et al. Sci Rep 2021; 11 (1) : 9749 Dietary cis-9, trans-11-conjugated linoleic acid reduces amyloid β-protein accumulation and upregulates anti-inflammatory cytokines in an Alzheimer's disease mouse model.
Application : IHC-Fr
Reactivity : Mouse
M?ndez-Lara KA et al. Antioxidants (Basel) 2020; 9 (11) Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Application : IHC-P
Reactivity : Mouse
Luo Q et al. Molecules 2019; 24 (6) Improvement of Colonic Immune Function with Soy Isoflavones in High-Fat Diet-Induced Obese Rats.
Application : WB
Reactivity : Rat
Singh AK et al. Sci Rep 2018; 8 (1) : 5932 Novel Indole-fused benzo-oxazepines (IFBOs) inhibit invasion of hepatocellular carcinoma by targeting IL-6 mediated JAK2/STAT3 oncogenic signals.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations