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IL10 antibody [GT5111]

Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359
Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359
Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359

Cat No. GTX632359

Host

Mouse

Clonality

Monoclonal

Clone Name

GT5111

Isotype

IgG1

Application

WB, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

3x Flag human IL-10-transfected 293T

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Guinea pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL10. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888308

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359

GTX632359 IHC-P Image

IL10 antibody [GT5111] detects IL10 protein in mouse thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
IL10 antibody [GT5111] (GTX632359) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359

GTX632359 WB Image

IL10 antibody [GT5111] detects IL10 protein by western blot analysis. Non-transfected (-) and IL-10-transfected (+, including 3x Flag-tag) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody [GT5111] (GTX632359) at a dilution of 1:5000.

Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359

GTX632359 IHC-P Image

IL10 antibody [GT5111] detects IL10 protein in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
IL10 antibody [GT5111] (GTX632359) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169