APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1/50-1/250 |
| 1/50-1/100 |
| Assay dependent |
| Assay dependent |
|
Note :
IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human IL-10 expressed by COS cells.
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF
Cellular Localization
Secreted
Background
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
Database
Research Area
DATA IMAGES
![Anti-IL10 antibody [JES3-12G8] used in Western Blot (WB). GTX39962](/templates/default/js/lightbox/images/loading.gif "Anti-IL10 antibody [JES3-12G8] used in Western Blot (WB). GTX39962")
|
GTX39962 WB Image
WB analysis of human IL-10 recombinant protein using GTX39962 IL10 antibody [JES3-12G8].
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REFERENCE
There are currently no references for IL10 antibody [JES3-12G8] (GTX39962). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL10 antibody [JES3-12G8] (GTX39962). Be the first to share your experience with this product.
![Anti-IL10 antibody [JES3-12G8] used in Western Blot (WB). GTX39962](/upload/website/prouct_img/small/GTX39962/GTX39962_3979_WB_18121410_778.jpg "Anti-IL10 antibody [JES3-12G8] used in Western Blot (WB). GTX39962")
![Anti-IL10 antibody [JES3-12G8] used in Western Blot (WB). GTX39962](/upload/website/prouct_img/normal/GTX39962/GTX39962_3979_WB_18121410_778.jpg "Anti-IL10 antibody [JES3-12G8] used in Western Blot (WB). GTX39962")