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IL10 antibody [JES3-9D7] (Low endotoxin, azide free)

Anti-IL10 antibody [JES3-9D7] (Low endotoxin, azide free) used in Western Blot (WB). GTX39969

Cat. No. GTX39969

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 JES3-9D7

Isotype

 IgG1

Application

 WB, FACS, ELISA, IHC, Neutralizing/Inhibition

Reactivity

 Human
Package
500 μg ($739)
See all IL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/100
FACS 1/10-1/50
ELISA Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Membrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Rat anti Human Interleukin-10 antibody, clone JES3-9D7 is reported to act as a neutralising antibody (Othieno et al. 1999).
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Product Note

Clone JES3-9D7 is reported to act as a neutralising antibody (Othieno et al. 1999).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-10.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_11164919

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-IL10 antibody [JES3-9D7] (Low endotoxin, azide free) used in Western Blot (WB). GTX39969

GTX39969 WB Image

WB analysis of human IL-10 recombinant protein using GTX39969 IL10 antibody [JES3-9D7] (Low endotoxin, azide free).

REFERENCE

Application Reference

Buommino E et al. Arch Dermatol Res 2009; 301 (6) : 397-404 Osteopontin: a new emerging role in psoriasis.
Application : IHC
Reactivity : Human

Clone Reference

Brodeur ND et al. Virus Res 2010; 153 (2):265-8 Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity.
Application : ELISA
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 739
Custom Conjugations