IL10 antibody

Cat No. GTX130513

Host	Rabbit
Clonality	Polyclonal
Application	WB, IHC-Fr, ELISA
Reactivity	Human, Mouse
Package	100 μl ($319), 25 μl ($169)

See all IL10 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
IHC-Fr	1:100-1:1000
ELISA	1:-1:10000

Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , human IL10-transfected 293T

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IL10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Interleukin 10,Csif,Gvhds,Il-10,Il10A,Tgif,Il10

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]

Database

Gene ID: 3586
UniProt: P22301

Research Area

DATA IMAGES

GTX130513 IHC-Fr Image

IL10 antibody detects IL10 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL10 stained by IL10 antibody (GTX130513) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX130513 ELISA Image

An ELISA plate is coated with 50 μL of a IL10 peptide at concentration ranged from 0.25 μg/mL to 8 μg/mL. The coated peptide is detected with anti IL10 antibody (GTX130513) at 0.5 μg/mL.

GTX130513 WB Image

IL10 antibody detects IL10 protein by western blot analysis. Non-transfected (-) and IL10-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody (GTX130513) diluted at 1:5000.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 319

25 μl

$ 169

Qty