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IL10 antibody

Anti-IL10 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130513
Anti-IL10 antibody used in Western Blot (WB). GTX130513
Anti-IL10 antibody used in ELISA (ELISA). GTX130513
Anti-IL10 antibody used in IHC (Paraffin sections) (IHC-P). GTX130513

Cat No. GTX130513

Reference 3
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($379),
25 μl ($169)
See all IL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
ELISA 1:-1:10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , human IL10-transfected 293T

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IL10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886295

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL10 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130513

GTX130513 IHC-Fr Image

IL10 antibody detects IL10 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL10 stained by IL10 antibody (GTX130513) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL10 antibody used in Western Blot (WB). GTX130513

GTX130513 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody (GTX130513) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL10 antibody used in ELISA (ELISA). GTX130513

GTX130513 ELISA Image

An ELISA plate is coated with 50 μL of a IL10 peptide at concentration ranged from 0.25 μg/mL to 8 μg/mL. The coated peptide is detected with anti IL10 antibody (GTX130513) at 0.5 μg/mL.

Anti-IL10 antibody used in IHC (Paraffin sections) (IHC-P). GTX130513

GTX130513 IHC-P Image

IL10 antibody detects IL10 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
IL10 stained by IL10 antibody (GTX130513) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Petrella C et al. Pharmacol Res 2021; 172 : 105795 Proneurogenic and neuroprotective effect of a multi strain probiotic mixture in a mouse model of acute inflammation: Involvement of the gut-brain axis.
Application : WB
Reactivity : Mouse
Zhou Y et al. Oncol Lett 2020; 20 (6) : 298 Anti-CD206 antibody-conjugated Fe3O4-based PLGA nanoparticles selectively promote tumor-associated macrophages to polarize to the pro-inflammatory subtype.
Application : WB
Reactivity : Mouse
Xiaopeng Tang et al. bioRxiv 2020; (Epub) Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

IL10 antibody Cat No. GTX130513

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-IL10 antibody used in Western Blot (WB). GTX130513
Date : Anonymous submitted on 22-Feb-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : colonic mucosa
Amount used : 25 μg
Blocking : 5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169