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IL10 antibody

Anti-IL10 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130513
Anti-IL10 antibody used in ELISA (ELISA). GTX130513
Anti-IL10 antibody used in Western Blot (WB). GTX130513

Cat No. GTX130513

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all IL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-Fr 1:100-1:1000
ELISA 1:-1:10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , human IL10-transfected 293T

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IL10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL10 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130513

GTX130513 IHC-Fr Image

IL10 antibody detects IL10 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL10 stained by IL10 antibody (GTX130513) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL10 antibody used in ELISA (ELISA). GTX130513

GTX130513 ELISA Image

An ELISA plate is coated with 50 μL of a IL10 peptide at concentration ranged from 0.25 μg/mL to 8 μg/mL. The coated peptide is detected with anti IL10 antibody (GTX130513) at 0.5 μg/mL.

Anti-IL10 antibody used in Western Blot (WB). GTX130513

GTX130513 WB Image

IL10 antibody detects IL10 protein by western blot analysis. Non-transfected (-) and IL10-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody (GTX130513) diluted at 1:5000.

REFERENCE

Application Reference

Xiaopeng Tang et al. bioRxiv 2020; (Epub) Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169