GeneTex
United States (US)

IL12 antibody [C8.6] (Biotin)

GTX35255_GTX35255_ELISA_Image_19012408_738.jpg

Cat No. GTX35255

Host Mouse
Clonality Monoclonal
Clone Name C8.6
Isotype IgG1
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody is specific for natural IL-12 and recognizes the p70 heterodimer as well as the p40 subunit (monomers and homodimers). It will not cross-react with the p35 subunit.
PROPERTIES

Form

Liquid

Buffer

PBS, 4% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

COS cell-derived recombinant human IL-12, p40 subunit

Purification

Protein A purified.

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
GTX35255_GTX35255_ELISA_Image_19012408_738.jpg

GTX35255 ELISA Image

Sandwich ELISA analysis of human IL12 protein in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/ml (50 ul per lane) using GTX79487 as capture antibody (7 ug/ml) and GTX35255 as detection antibody (0.03125 ug/ml). The plate was washed then incubated with Streptavidin-HRP. Detection was performed with TMB substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.

Package List Price ($)
$ 329