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IL12 antibody [C8.6] (Biotin)

Anti-IL12 antibody [C8.6] (Biotin) used in ELISA (ELISA). GTX35255

Cat. No. GTX35255

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C8.6

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
250 μg ($409)
See all IL12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Product Note

This antibody is specific for natural IL-12 and recognizes the p70 heterodimer as well as the p40 subunit (monomers and homodimers). It will not cross-react with the p35 subunit.

PROPERTIES

Form

Liquid

Buffer

PBS, 4% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

COS cell-derived recombinant human IL-12, p40 subunit

Purification

Protein A purified.

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 12A , interleukin 12B , CLMF , IL-12A , NFSK , NKSF1 , P35 , CLMF , CLMF2 , IL-12B , IMD28 , IMD29 , NKSF , NKSF2

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES

Anti-IL12 antibody [C8.6] (Biotin) used in ELISA (ELISA). GTX35255

GTX35255 ELISA Image

Sandwich ELISA analysis of human IL12 protein in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/ml (50 ul per lane) using GTX79487 as capture antibody (7 ug/ml) and GTX35255 as detection antibody (0.03125 ug/ml). The plate was washed then incubated with Streptavidin-HRP. Detection was performed with TMB substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 409