GeneTex
United States (US)

IL12 antibody [CC301] (Low endotoxin, azide free)

Cat No. GTX39960

Host Mouse
Clonality Monoclonal
Clone Name CC301
Isotype IgG2a
Application WB, FACS, ELISA, Functional Assay, ELISPOT
Reactivity Human, Sheep, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/100
FACS 1/10
ELISA 8μg/ml
Functional Assay 1/100
ELISPOT Assay dependent

Note :

Membrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Specificity/Sensitivity

This antibody recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer.This antibody may be used as a capture antibody in an ELISA in combination with biotinylated clone CC326 as a detection reagent.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0mg/ml

Antigen Species

Bovine

Immunogen

Recombinant bovine IL-12.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
SDS
PBS.pdf