Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/100 |
1/10 |
8μg/ml |
Assay dependent |
Assay dependent |
Note :
FACS
Membrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
|
Not tested in other applications.
Product Note
This antibody recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer.This antibody may be used as a capture antibody in an ELISA in combination with biotinylated clone CC326 as a detection reagent.
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Bovine
Immunogen
Recombinant bovine IL-12.
Purification
Protein A purified
From tissue culture supernatant
Endotoxin
< 0.01 EU/μg (determined by the LAL assay)
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.