IL12A / IL12 p35 antibody, C-term

Cat. No. GTX81020

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS
Reactivity	Human
Package	400 μl ($399)

See all IL12A / IL12 p35 products

Datasheet
Document

View product citations for antibody GTX81020 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
IHC-P	1:10-1:50
FACS	1:10-1:50

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 168-195 amino acids from the C-terminal region of human IL12A.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 12A , CLMF , IL-12A , NFSK , NKSF1 , P35

Cellular Localization

Secreted

Background

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3592 IL12A
UniProt: P29459 IL12A

Research Area

DATA IMAGES

GTX81020 WB Image

WB analysis of MDA-MB231 cell lysate (35ug/lane) using GTX81020 IL12A / IL12 p35 antibody, C-term.

GTX81020 IHC-P Image

IHC-P analysis of human breast carcinoma using GTX81020 IL12A / IL12 p35 antibody, C-term.

GTX81020 FACS Image

FACS analysis of MDA-MB231 cells using GTX81020 IL12A / IL12 p35 antibody, C-term.
Top histogram : negative control
Bottom histogram : MDA-MB231 cells

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

400 μl

$ 399

Qty