IL12B / IL12 p40 antibody [C17-8] (APC)

Cat. No. GTX01491-07

Host	Rat
Clonality	Monoclonal
Clone Name	C17-8
Isotype	IgG2a
Application	FACS
Reactivity	Mouse
Package	100 μg ($349)

See all IL12B / IL12 p40 products

View product citations for antibody GTX01491-07 on CiteAb

PRODUCT

Summary

The C17.8 antibody is specific for the 40 kDa (p40) protein subunit shared by the cytokines IL-12 and IL-23. To form IL-12, p40 assembles with a separate 35 kDa protein known as p35, resulting in a 70 kDa functional cytokine. IL-12 is secreted by activated monocytes, macrophages, and dendritic cells, and has been shown to target naive, resting CD4+ T cells to promote their proliferation and secretion of cytokines. IL-23 contains the p40 subunit in combination with a 19 kDa protein chain, p19; its primary source being activated dendritic cells and other antigen-presenting cells. IL-23 appears to target different cell types than IL-12, acting on memory CD4+ T cells to induce a strong proliferative response and contributing to the generation and expansion of Th17 cells. Like the cytokines themselves, the receptors for IL-12 and IL-23 share one subunit, as well as containing distinct cytokine-specific subunits.As the C17.8 antibody binds to a shared subunit of both IL-12 and IL-23, it may be used as a marker for either IL-12 or IL-23 expression in dendritic cells, monocytes and macrophages, and is widely used for neutralization of activity associated with either cytokine.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 12a , interleukin 12b , IL-12p35 , Il-12a , Ll12a , p35 , Il-12b , Il-12p40 , Il12p40 , p40

Cellular Localization

Secreted

Database

Gene ID: 16160 Il12a
UniProt: P43431 Il12a
UniProt: P43432 Il12b

Research Area

DATA IMAGES

GTX01491-07 FACS Image

FACS analysis of mouse macrophages (stimulated in the presence of a protein transport inhibitor) using GTX01491-07 IL23 antibody [C17.8] (APC).
Right panel : primary antibody
Left panel : isotype control
antibody amount : 0.06μg (5 μl)

REFERENCE

Clone Reference

Su Y et al. Clin Exp Immunol 2017; (Epub) Withasteroid B from D. metel L. Regulates Immune Responses by Modulating the JAK/STAT Pathway and the IL-17⁺ RORγt⁺ /IL-10⁺ FoxP3⁺ Ratio.

2012; CD27 stimulation promotes the frequency of IL-7 receptor-expressing memory precursors and prevents IL-12-mediated loss of CD8(+) T cell memory in the absence of CD4(+) T cell help.

2011; Suppression of the inflammatory immune response prevents the development of chronic biofilm infection due to methicillin-resistant Staphylococcus aureus.

2011; IL-12 release by engineered T cells expressing chimeric antigen receptors can effectively Muster an antigen-independent macrophage response on tumor cells that have shut down tumor antigen expression.

2003; Antitumor and antimetastatic activity of IL-23.

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REVIEW

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Clone Reference

Su Y et al. Clin Exp Immunol 2017; (Epub) Withasteroid B from D. metel L. Regulates Immune Responses by Modulating the JAK/STAT Pathway and the IL-17⁺ RORγt⁺ /IL-10⁺ FoxP3⁺ Ratio.

2012; CD27 stimulation promotes the frequency of IL-7 receptor-expressing memory precursors and prevents IL-12-mediated loss of CD8(+) T cell memory in the absence of CD4(+) T cell help.

2011; Suppression of the inflammatory immune response prevents the development of chronic biofilm infection due to methicillin-resistant Staphylococcus aureus.

2003; Antitumor and antimetastatic activity of IL-23.

2002; IL-23 and IL-12 have overlapping, but distinct, effects on murine dendritic cells.

1999; Role of IL-12 in intrathymic negative selection.

1996; Local production of the p40 subunit of interleukin 12 suppresses T-helper 1-mediated immune responses and prevents allogeneic myoblast rejection.

1995; Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice.