APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture: GTX02957, Detection: GTX02956-02 (MT618 for p40) / GTX03013-02 (MT704 for p70) / GTX02983-02.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody is able to detect native and recombinant human p40 subunit of IL-12 and IL-23. In combination with MT86/221, it is able to recognize p40 monomer, p40 homodimer, IL-12 p70 heterodimer (p40 + p35) and IL-23 heterodimer (p40 + p19).
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human IL-23
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CLMF,CLMF2,IL12B,IMD28,IMD29,NKSF,NKSF2,interleukin 12B,IL12 p40
Cellular Localization
Secreted
Background
This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-IL12B / IL12 p40 antibody [MT618] (Biotin) used in ELISA (ELISA). GTX02956-02](/templates/default/js/lightbox/images/loading.gif "Anti-IL12B / IL12 p40 antibody [MT618] (Biotin) used in ELISA (ELISA). GTX02956-02")
|
GTX02956-02 ELISA Image
Sandwich ELISA analysis of human IL-12/-23 (p40) protein using GTX02957 IL12B / IL12 p40 antibody [MT86 + MT221] as coating antibody and GTX02956-02 IL12B / IL12 p40 antibody [MT618] (Biotin) as detecting antibody.
Substrate : pNPP
|
REFERENCE
There are currently no references for IL12B / IL12 p40 antibody [MT618] (Biotin) (GTX02956-02). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL12B / IL12 p40 antibody [MT618] (Biotin) (GTX02956-02). Be the first to share your experience with this product.
![Anti-IL12B / IL12 p40 antibody [MT618] (Biotin) used in ELISA (ELISA). GTX02956-02](/upload/website/prouct_img/small/GTX02956-02/GTX02956-02_20210507_ELISA_w_23053123_808.webp "Anti-IL12B / IL12 p40 antibody [MT618] (Biotin) used in ELISA (ELISA). GTX02956-02")
![Anti-IL12B / IL12 p40 antibody [MT618] (Biotin) used in ELISA (ELISA). GTX02956-02](/upload/website/prouct_img/normal/GTX02956-02/GTX02956-02_20210507_ELISA_w_23053123_808.webp "Anti-IL12B / IL12 p40 antibody [MT618] (Biotin) used in ELISA (ELISA). GTX02956-02")