GeneTex
United States (US)

IL13 Receptor alpha 1 antibody

Orthogonal Overexpression
GTX105788_40401_IHC_19011506_319.jpg
GTX105788_42795_20180727_WB_B_19011506_642.jpg
GTX105788_42795_20171020_WB_Fraction_19011506_215.jpg

Cat No. GTX105788

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

U87-MG , U87-MG membrane extract , IL13RA1-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.51mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL13 Receptor alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 13 Receptor Subunit Alpha 1,Cd213A1,Ct19,Il-13Ra,Nr4,Il13Ra1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. [provided by RefSeq]

Database

DATA IMAGES
GTX105788_40401_IHC_19011506_319.jpg

GTX105788 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using IL13 Receptor alpha 1(GTX105788) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105788_42795_20180727_WB_B_19011506_642.jpg

GTX105788 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL13 Receptor alpha 1 antibody (GTX105788) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105788_42795_20171020_WB_Fraction_19011506_215.jpg

GTX105788 WB Image

Orthogonal

U87-MG whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL13 Receptor alpha 1 antibody (GTX105788) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169