GeneTex
United States (US)

IL16 antibody [C3], C-term

GTX108425_WB_19011507_872.jpg
GTX108425_39848_20141205_IHC_19011507_516.jpg
GTX108425_39848_IFA_19011507_442.jpg

Cat No. GTX108425

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

A549 , Jarkat , NT2D1

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IL16. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 16,Lcf,Nil16,Pril16,Pril-16,Il16

Cellular Localization

Interleukin-16: Secreted , Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Nucleus

Background

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES
GTX108425_WB_19011507_872.jpg

GTX108425 WB Image

Sample (30 ug of whole cell lysate)
A: A549
5% SDS PAGE
IL16 antibody
GTX108425 diluted at 1:1000

GTX108425_39848_20141205_IHC_19011507_516.jpg

GTX108425 IHC-P Image

IL16 antibody [C3], C-term detects IL16 protein at secreted on human normal colon by immunohistochemical analysis.
Sample: Paraffin-embedded human normal colon.
IL16 antibody [C3], C-term (GTX108425) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108425_39848_IFA_19011507_442.jpg

GTX108425 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using IL16(GTX108425) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169