GeneTex
United States (US)

IL17A antibody

GTX32674_0052790201_20190201_WB_R_spleen_19022001_641.jpg
GTX32674_20181108_WB_19011519_884.jpg

Cat No. GTX32674

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200 - 1:500
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-155 of human IL17A (NP_002181.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 17A,Ctla-8,Ctla8,Il-17,Il-17A,Il17,Il17A

Background

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32674_0052790201_20190201_WB_R_spleen_19022001_641.jpg

GTX32674 WB Image

Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with IL17A antibody (GTX32674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX32674_20181108_WB_19011519_884.jpg

GTX32674 WB Image

WB analysis of mouse and rat liver tissue lysate using GTX32674 IL17A antibody.

REVIEW

IL17A antibody Cat No. GTX32674

Rating ( Average 3 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2017
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Spleen
Blocking : 1XPBS+ 0.3% Triton? X-100 + 1%BSA+human serum, 25°C, 0.5Hr
Primary Antibody : 1:30, 4°C, overnightHr
Package List Price ($)
$ 319