*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:2,000
|1:1,000 - 1:5,000
Not tested in other applications.
In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-F in cell supernatants and certain body fluids.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-F protein.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
interleukin 17F , CANDF6 , IL-17F , ML-1 , ML1
IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1.
GTX48695 WB Image
Western blot using GeneTex's anti-Human IL17F antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL17F (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer). Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).