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IL17F antibody

Anti-IL17F antibody used in Western Blot (WB). GTX48695

Cat No. GTX48695

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2,000
ELISA 1:1,000 - 1:5,000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Specificity/Sensitivity

In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-F in cell supernatants and certain body fluids.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-F protein.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 17F , CANDF6 , IL-17F , ML-1 , ML1

Cellular Localization

Secreted

Background

IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1.

Database

Research Area

DATA IMAGES

Anti-IL17F antibody used in Western Blot (WB). GTX48695

GTX48695 WB Image

Western blot using GeneTex's anti-Human IL17F antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL17F (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer). Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

REFERENCE

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