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IL17F antibody

Anti-IL17F antibody used in Western Blot (WB). GTX48759
Anti-IL17F antibody used in Western Blot (WB). GTX48759

Cat. No. GTX48759

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA

Reactivity

 Mouse
Package
50 μg ($399)
See all IL17F products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
Dot Assay dependent
ELISA 1:10000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Full length recombinant mouse IL17-F protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 17F , C87042 , IL-17F

Cellular Localization

Secreted

Background

IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Database

Research Area

DATA IMAGES

Anti-IL17F antibody used in Western Blot (WB). GTX48759

GTX48759 WB Image

Western blot using GeneTex's anti-Mouse IL-17F antibody shows detection of a band ~18 kDa in size corresponding to recombinant mouse IL-17F. Recombinant mouse IL-17F was loaded on to an SDS-PAGE gel at 0.25 μg and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4ºC. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer) for 30 min at RT followed by reation with FemtoMax Chemiluminescent substrate. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.

Anti-IL17F antibody used in Western Blot (WB). GTX48759

GTX48759 WB Image

WB analysis of recombinant mouse IL17F protein using GTX48759 IL17F antibody.
Loading : 0.25 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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Package List Price ($)
$ 399