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IL17F antibody

Anti-IL17F antibody used in Western Blot (WB). GTX48759

Cat No. GTX48759

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Mouse, Rat
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ELISA 1:10,000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Specificity/Sensitivity

In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse IL17-F in cell supernatants and certain body fluids.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 17F , C87042 , IL-17F

Cellular Localization

Secreted

Background

IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Database

Research Area

DATA IMAGES

Anti-IL17F antibody used in Western Blot (WB). GTX48759

GTX48759 WB Image

Western blot using GeneTex's anti-Mouse IL-17F antibody shows detection of a band ~18 kDa in size corresponding to recombinant mouse IL-17F. Recombinant mouse IL-17F was loaded on to an SDS-PAGE gel at 0.25 μg and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4ºC. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer) for 30 min at RT followed by reation with FemtoMax Chemiluminescent substrate. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.

REFERENCE

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REVIEW

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