Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1761] (GTX637411) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below.

Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL)
Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL)
Please notice that needs to be conjugated to HRP to function as the detection antibody when paired with . Please contact us for custom HRP-conjugated antibody.

Human IL18 protein was separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1761] (GTX637411) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1761] (GTX637411) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1761] (GTX637411) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

IL18 antibody [HL1761] detects IL18 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
IL18 stained by IL18 antibody [HL1761] (GTX637411) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min