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IL18 antibody [HL1859]

Recombinant antibody Orthogonal
Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584
Anti-IL18 antibody [HL1859] used in IHC (Paraffin sections) (IHC-P). GTX637584
Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584
Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584
Anti-IL18 antibody [HL1859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637584
Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584
Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584

Cat. No. GTX637584

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1859

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IL18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX637584, Detection : GTX637411 or Capture : GTX637411, Detection : GTX637584.
The detection antibody requires HRP conjugation. Please contact us for the conjugation service.
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant fragment of mouse IL18

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 18 , Igif , Il-18

Cellular Localization

Secreted

Background

Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18). [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584

GTX637584 ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below.

Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL)
Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL)
Please notice that needs to be conjugated to HRP to function as the detection antibody when paired with . Please contact us for custom HRP-conjugated antibody.

Anti-IL18 antibody [HL1859] used in IHC (Paraffin sections) (IHC-P). GTX637584

GTX637584 IHC-P Image

IL18 antibody [HL1859] detects secreted IL18 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
IL18 stained by IL18 antibody [HL1859] (GTX637584) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584

GTX637584 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1859] (GTX637584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584

GTX637584 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1859] (GTX637584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL18 antibody [HL1859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637584

GTX637584 ICC/IF Image

IL18 antibody [HL1859] detects IL18 protein at Golgi apparatus and cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL18 stained by IL18 antibody [HL1859] (GTX637584) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584

GTX637584 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1859] (GTX637584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584

GTX637584 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1859] (GTX637584) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations