IL18 antibody [HL1859]

Cat. No. GTX637584
Cat. No.  GTX637584
Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584
GTX637584 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1859] (GTX637584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

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Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584
GTX637584 ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below.

Capture: IL18 antibody [HL2938] (GTX640322) (5 μg/mL)
Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584) (1 μg/mL)
Please notice that GTX637584 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640322. Please contact us for custom HRP-conjugated antibody.

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Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584
GTX637584 ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below.

Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL)
Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL)
Please notice that needs to be conjugated to HRP to function as the detection antibody when paired with . Please contact us for custom HRP-conjugated antibody.

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Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584
GTX637584 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1859] (GTX637584) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

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Anti-IL18 antibody [HL1859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637584
GTX637584 ICC/IF Image

IL18 antibody [HL1859] detects IL18 protein at Golgi apparatus and cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL18 stained by IL18 antibody [HL1859] (GTX637584) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584
GTX637584 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1859] (GTX637584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584
GTX637584 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1859] (GTX637584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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Anti-IL18 antibody [HL1859] used in IHC (Paraffin sections) (IHC-P). GTX637584
GTX637584 IHC-P Image

IL18 antibody [HL1859] detects secreted IL18 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
IL18 stained by IL18 antibody [HL1859] (GTX637584) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584
Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584
Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584
Anti-IL18 antibody [HL1859] used in ELISA (ELISA). GTX637584
Anti-IL18 antibody [HL1859] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637584
Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584
Anti-IL18 antibody [HL1859] used in Western Blot (WB). GTX637584
Anti-IL18 antibody [HL1859] used in IHC (Paraffin sections) (IHC-P). GTX637584
  • Host
    Rabbit
  • Clonality
    Monoclonal
  • Clone Name
    HL1859
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P ELISA Sandwich ELISA
  • Reactivity
    Human, Mouse
See all IL18 products