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IL18 antibody

KO/KD
Anti-IL18 antibody used in Western Blot (WB). GTX32675
Anti-IL18 antibody used in Immunoprecipitation (IP). GTX32675
Anti-IL18 antibody used in Western Blot (WB). GTX32675

Cat. No. GTX32675

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all IL18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P Assay dependent
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 37-193 of human IL18 (NP_001553.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 18 , IGIF , IL-18 , IL-1g , IL1F4

Cellular Localization

Secreted

Background

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IL18 antibody used in Western Blot (WB). GTX32675

GTX32675 WB Image

WB analysis of various sample lysates using GTX32675 IL18 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IL18 antibody used in Immunoprecipitation (IP). GTX32675

GTX32675 IP Image

IP analysis of HeLa cell lysate using GTX32675 IL18 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-IL18 antibody used in Western Blot (WB). GTX32675

GTX32675 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32675 IL18 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Stock AJ et al. PLoS Genet 2022; 18 (11) : e1010506 Aberrant expression and localization of the RAP1 shelterin protein contribute to age-related phenotypes.
Shiyu Xia et al. Nature 2021; 593 (7860) : 607-611 Gasdermin D pore structure reveals preferential release of mature interleukin-1.
Application : WB
Reactivity : Human
Zhang C et al. Mol Med Rep 2021; 23 (5) Cytokine secretion and pyroptosis of cholesteatoma keratinocytes mediated by AIM2 inflammasomes in response to cytoplasmic DNA.
Application : WB, IHC-P
Reactivity : Human
Chen J et al. Free Radic Biol Med 2020; (Epub) Low shear stress induced vascular endothelial cell pyroptosis by TET2/SDHB/ROS pathway.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399