HepG2 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] (GTX104513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IL1RAP antibody [N1N3] detects IL1RAP protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IL1RAP stained by IL1RAP antibody [N1N3] (GTX104513) diluted at 1:300.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] (GTX104513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.