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IL1RAP antibody [N1N3]

Orthogonal
Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513
Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513
Anti-IL1RAP antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX104513

Cat. No. GTX104513

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Pig
Package
100 μl ($399),
25 μl ($169)
See all IL1RAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

mouse liver , HepG2 membrane extract

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human IL1RAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172994

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 receptor accessory protein , C3orf13 , IL-1RAcP , IL1R3

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Secreted , Isoform 3: Secreted

Background

Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. This gene encodes an interleukin 1 receptor accessory protein. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513

GTX104513 WB Image

Orthogonal

HepG2 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] (GTX104513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1RAP antibody [N1N3] used in Western Blot (WB). GTX104513

GTX104513 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IL1RAP antibody [N1N3] (GTX104513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL1RAP antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX104513

GTX104513 IHC-P Image

IL1RAP antibody [N1N3] detects IL1RAP protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IL1RAP stained by IL1RAP antibody [N1N3] (GTX104513) diluted at 1:300.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zhao X et al. Mol Cell Proteomics 2017; (Epub) microRNA-4331 promotes TGEV-induced mitochondrial damage via targeting RB1, up-regulating IL1RAP, and activating p38 MAPK pathway in vitro.
Application : WB
Reactivity : Pig
Seo H et al. Biol Reprod 2012; 87 (2) : 31 Regulatory mechanism for expression of IL1B receptors in the uterine endometrium and effects of IL1B on prostaglandin synthetic enzymes during the implantation period in pigs.
Application : WB
Reactivity : Pig
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169