APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/500 - 1/2000 |
1/200 - 1/1000 |
1/200 - 1/1000 |
1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 2 receptor subunit alpha , CD25 , IDDM10 , IL2R , IMD41 , TCGFR , p55
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
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GTX60792 WB Image
WB analysis of HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate using GTX60792 IL2 Receptor alpha antibody [1B5D12].
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GTX60792 IHC-P Image
IHC-P analysis of bladder cancer tissue using GTX60792 IL2 Receptor alpha antibody [1B5D12].
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GTX60792 ELISA Image
ELISA analysis of antigen using GTX60792 IL2 Receptor alpha antibody [1B5D12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60792 WB Image
WB analysis of HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH3T3 (9) cell lysate using GTX60792 IL2 Receptor alpha antibody [1B5D12].
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GTX60792 IHC-P Image
IHC-P analysis of rectum cancer tissue using GTX60792 IL2 Receptor alpha antibody [1B5D12].
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GTX60792 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60792 IL2 Receptor alpha antibody [1B5D12]. Green : IL2 Receptor alpha Blue: DRAQ5 fluorescent DNA dye
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REFERENCE
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REVIEW
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