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IL2 Receptor alpha antibody [1B5D12]

Anti-IL2 Receptor alpha antibody [1B5D12] used in ELISA (ELISA). GTX60792
Anti-IL2 Receptor alpha antibody [1B5D12] used in IHC (Paraffin sections) (IHC-P). GTX60792
Anti-IL2 Receptor alpha antibody [1B5D12] used in Western Blot (WB). GTX60792
Anti-IL2 Receptor alpha antibody [1B5D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60792
Anti-IL2 Receptor alpha antibody [1B5D12] used in Western Blot (WB). GTX60792
Anti-IL2 Receptor alpha antibody [1B5D12] used in IHC (Paraffin sections) (IHC-P). GTX60792

Cat No. GTX60792

Host

Mouse

Clonality

Monoclonal

Clone Name

1B5D12

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 2 receptor subunit alpha , CD25 , IDDM10 , IL2R , IMD41 , TCGFR , p55

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IL2 Receptor alpha antibody [1B5D12] used in ELISA (ELISA). GTX60792

GTX60792 ELISA Image

ELISA analysis of antigen using GTX60792 IL2 Receptor alpha antibody [1B5D12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-IL2 Receptor alpha antibody [1B5D12] used in IHC (Paraffin sections) (IHC-P). GTX60792

GTX60792 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60792 IL2 Receptor alpha antibody [1B5D12].

Anti-IL2 Receptor alpha antibody [1B5D12] used in Western Blot (WB). GTX60792

GTX60792 WB Image

WB analysis of HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH3T3 (9) cell lysate using GTX60792 IL2 Receptor alpha antibody [1B5D12].

Anti-IL2 Receptor alpha antibody [1B5D12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60792

GTX60792 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60792 IL2 Receptor alpha antibody [1B5D12].
Green : IL2 Receptor alpha
Blue: DRAQ5 fluorescent DNA dye

Anti-IL2 Receptor alpha antibody [1B5D12] used in Western Blot (WB). GTX60792

GTX60792 WB Image

WB analysis of HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate using GTX60792 IL2 Receptor alpha antibody [1B5D12].

Anti-IL2 Receptor alpha antibody [1B5D12] used in IHC (Paraffin sections) (IHC-P). GTX60792

GTX60792 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60792 IL2 Receptor alpha antibody [1B5D12].

REFERENCE

There are currently no references for IL2 Receptor alpha antibody [1B5D12] (GTX60792). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IL2 Receptor alpha antibody [1B5D12] (GTX60792). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339