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IL2 Receptor alpha antibody [BC96] (APC)

Anti-IL2 Receptor alpha antibody [BC96] (APC) used in Flow cytometry (FACS). GTX01471-07

Cat No. GTX01471-07

Host

Mouse

Clonality

Monoclonal

Clone Name

BC96

Isotype

IgG1

Application

FACS

Reactivity

Human, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package
100 test ($199)
PRODUCT

Summary

The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells. The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on “induced” peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 0.125 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.025 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 2 receptor subunit alpha , CD25 , IDDM10 , IL2R , IL2RA , IMD41 , TCGFR , p55 , IL2-R alpha

Cellular Localization

Membrane

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IL2 Receptor alpha antibody [BC96] (APC) used in Flow cytometry (FACS). GTX01471-07

GTX01471-07 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01471-07 IL2 Receptor alpha antibody [BC96] (APC).
Right panel : co-stained with IL2 Receptor alpha antibody [BC96] (APC) and Human CD4 antibody (FITC)
Left panel : co-stained with isotype control and Human CD4 antibody (FITC)
antibody amount : 0.125 μg (5 μl)

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 199