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IL2 Receptor alpha antibody [BC96] (PE-Cy7)

Anti-IL2 Receptor alpha antibody [BC96] (PE-Cy7) used in Flow cytometry (FACS). GTX01471-10

Cat No. GTX01471-10

Host

Mouse

Clonality

Monoclonal

Clone Name

BC96

Isotype

IgG1

Application

FACS

Reactivity

Human, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package
100 test ($239)
PRODUCT

Summary

The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells. The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on “induced” peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin-Cyanine7 (PE-Cy7)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 2 receptor subunit alpha , CD25 , IDDM10 , IL2R , IL2RA , IMD41 , TCGFR , p55 , IL2-R alpha

Cellular Localization

Membrane

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IL2 Receptor alpha antibody [BC96] (PE-Cy7) used in Flow cytometry (FACS). GTX01471-10

GTX01471-10 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01471-10 IL2 Receptor alpha antibody [BC96] (PE-Cy7).
Right panel : co-stained with IL2 Receptor alpha antibody [BC96] (PE-Cy7) and Human CD4 antibody (FITC)
Left panel : co-stained with isotype control and Human CD4 antibody (FITC)
antibody amount : 0.25 μg (5 μl)

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 239