APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2-4μg/ml for 30 minutes at RT |
| Assay dependent |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human IL2RA protein (around aa 42-183) (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD25 , IDDM10 , IL2R , IL2RA , IMD41 , TCGFR , interleukin 2 receptor subunit alpha , p55 , IL2-R alpha
Cellular Localization
Membrane
Background
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
![Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115](/templates/default/js/lightbox/images/loading.gif "Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115")
|
GTX18115 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX18115 IL2 Receptor alpha antibody [IL2RA/2395].
|
|
|
GTX18115 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD25 Mouse Monoclonal Antibody (IL2RA/2395). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
|
|
|
GTX18115 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX18115 IL2 Receptor alpha antibody [IL2RA/2395].
|
REFERENCE
There are currently no references for IL2 Receptor alpha antibody [IL2RA/2395] (GTX18115). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL2 Receptor alpha antibody [IL2RA/2395] (GTX18115). Be the first to share your experience with this product.
![Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115](/upload/website/prouct_img/small/GTX18115/GTX18115_20200115_IHC-P_1295_w_23060620_443.webp "Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115")
![Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in Protein Array (Protein Array). GTX18115](/upload/website/prouct_img/small/GTX18115/GTX18115_20200115_Protein-Array_1924_w_23060620_508.webp "Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in Protein Array (Protein Array). GTX18115")
![Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115](/upload/website/prouct_img/small/GTX18115/GTX18115_20200115_IHC-P_1294_w_23060620_923.webp "Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115")
![Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115](/upload/website/prouct_img/normal/GTX18115/GTX18115_20200115_IHC-P_1295_w_23060620_443.webp "Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115")
![Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in Protein Array (Protein Array). GTX18115](/upload/website/prouct_img/normal/GTX18115/GTX18115_20200115_Protein-Array_1924_w_23060620_508.webp "Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in Protein Array (Protein Array). GTX18115")
![Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115](/upload/website/prouct_img/normal/GTX18115/GTX18115_20200115_IHC-P_1294_w_23060620_923.webp "Anti-IL2 Receptor alpha antibody [IL2RA/2395] used in IHC (Paraffin sections) (IHC-P). GTX18115")