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IL2 Receptor alpha antibody [MEM-181] (FITC)

Anti-IL2 Receptor alpha antibody [MEM-181] (FITC) used in Flow cytometry (FACS). GTX18275

Cat. No. GTX18275

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-181

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
100 test ($329)
See all IL2 Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PHA-activated peripheral blood leucocytes

Purification

Purified by size-exclusion chromatography

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD25 , IDDM10 , IL2R , IL2RA , IMD41 , TCGFR , interleukin 2 receptor subunit alpha , p55 , IL2-R alpha

Cellular Localization

Membrane

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IL2 Receptor alpha antibody [MEM-181] (FITC) used in Flow cytometry (FACS). GTX18275

GTX18275 FACS Image

FACS analysis of human PBMC cells using GTX18275 IL2 Receptor alpha antibody [MEM-181] (FITC). The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days.
Panel A, C : staining with GTX18275
Panel B, D : staining with a positive control anti-CD25 monoclonal antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329