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IL2 Receptor alpha antibody [MEM-181]

Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869
Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869
Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869

Cat No. GTX75869

Host Mouse
Clonality Monoclonal
Clone Name MEM-181
Isotype IgG1
Application FACS
Reactivity Human
Package
100 μg ($239)
APPLICATION

Application Note

Flow Cytometry: Use 10μl of the suggested working dilution to label 10^6 cells in 100μl. This antibody is not suitable for Western blot application. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

31 kDa. ( Note )

Specificity/Sensitivity

human CD25
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH7.4 containing 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human PHA blasts, day 3 of culture

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 2 Receptor Subunit Alpha,Cd25,Iddm10,Il2R,Imd41,Tcgfr,P55,Il2Ra

Background

Recognizes the 55kD chain of the IL-2 receptor, expressed by activated lymphocytes.

Database

Research Area

DATA IMAGES
Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869

GTX75869 FACS Image

Staining of PHA stimulated human lymphocyte cells with Mouse anti Human CD25 (GTX75869)

Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869

GTX75869 FACS Image

Surface staining of human PBMC with anti-human CD25: FITC (GTX75869). The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days.
Panel A, C – staining with anti-human CD25 (MEM-181) (GTX75869)
Panel B, D – staining with a standard anti-CD25 monoclonal antibody

Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869

GTX75869 FACS Image

Staining of PHA stimulated human peripheral blood lymphocytes with Mouse anti Human CD25:FITC (GTX18275)

REFERENCE
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REVIEW
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Package List Price ($)
$ 239