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IL2 Receptor alpha antibody [MEM-181]

Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869
Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869
Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869

Cat. No. GTX75869

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-181

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
100 μg ($339)
See all IL2 Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PHA-activated peripheral blood leucocytes

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_377084

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD25 , IDDM10 , IL2R , IL2RA , IMD41 , TCGFR , interleukin 2 receptor subunit alpha , p55 , IL2-R alpha

Cellular Localization

Membrane

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869

GTX75869 FACS Image

Staining of PHA stimulated human lymphocyte cells with Mouse anti Human CD25 (GTX75869)

Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869

GTX75869 FACS Image

Staining of PHA stimulated human peripheral blood lymphocytes with Mouse anti Human CD25:FITC (GTX18275)

Anti-IL2 Receptor alpha antibody [MEM-181] used in Flow cytometry (FACS). GTX75869

GTX75869 FACS Image

Surface staining of human PBMC with anti-human CD25: FITC (GTX75869). The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days.
Panel A, C – staining with anti-human CD25 (MEM-181) (GTX75869)
Panel B, D – staining with a standard anti-CD25 monoclonal antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339