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IL2 Receptor alpha antibody [PC61.5] (APC-Cy7)

Anti-IL2 Receptor alpha antibody [PC61.5] (APC-Cy7) used in Flow cytometry (FACS). GTX01472-15

Cat No. GTX01472-15

Host

Rat

Clonality

Monoclonal

Clone Name

PC61.5

Isotype

IgG1

Application

FACS

Reactivity

Mouse
Package
100 μg ($329)
PRODUCT

Summary

The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from naïve or conventional T cells which are CD25. This clone has also been reported for depletion of Treg cells in vivo.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄ (pH7.2), 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin-Cyanine7 (APC-Cy7)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Membrane

Database

Research Area

DATA IMAGES

Anti-IL2 Receptor alpha antibody [PC61.5] (APC-Cy7) used in Flow cytometry (FACS). GTX01472-15

GTX01472-15 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01472-15 IL2 Receptor alpha antibody [PC61.5] (APC-Cy7).
Right panel : co-stained with IL2 Receptor alpha antibody [PC61.5] (APC-Cy7) and Mouse CD4 antibody (PerCP-Cy5.5)
Left panel : co-stained with isotype control and Mouse CD4 antibody (PerCP-Cy5.5)
antibody amount : 0.25 μg (5 μl)

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 329