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IL2 Receptor alpha antibody [PC61.5]

Cat No. GTX01472

Host

Rat

Clonality

Monoclonal

Clone Name

PC61.5

Isotype

IgG1

Application

IHC-Fr, FACS, IP, Depletion

Reactivity

Mouse
Package
100 μg ($119)
PRODUCT

Summary

The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from naïve or conventional T cells which are CD25. This clone has also been reported for depletion of Treg cells in vivo.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Depletion Assay dependent
Inhibition assay Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄ (pH7.2), 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Purity

> 90% (determined by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Membrane

Database

Research Area

REFERENCE

REVIEW

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Clone Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 119