GeneTex
United States (US)

IL2 Receptor beta antibody [C3], C-term

GTX108495_39812_IHC_19011507_529.jpg
GTX108495_39812_IFA_19011507_367.jpg
GTX108495_WB_19011507_418.jpg

Cat No. GTX108495

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IL2 Receptor beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD122 antibody, IL15RB antibody, P70-75 antibody, IL2RB antibody, IL-2 receptor subunit beta antibody, IL-2RB antibody, interleukin-2 receptor subunit beta antibody, high affinity IL-2 receptor subunit beta antibody, high affinity IL-2 receptor beta subunit antibody, p75 antibody, CD122 antigen antibody, IL-2R subunit beta antibody, interleukin 15 receptor, beta antibody, interleukin 2 receptor, beta antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. [provided by RefSeq]

Database

DATA IMAGES
GTX108495_39812_IHC_19011507_529.jpg

GTX108495 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using IL2 Receptor beta(GTX108495) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108495_39812_IFA_19011507_367.jpg

GTX108495 ICC/IF Image

IL2 Receptor beta antibody [C3], C-term detects IL2RB protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL2RB protein stained by IL2 Receptor beta antibody [C3], C-term (GTX108495) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX108495_WB_19011507_418.jpg

GTX108495 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
7.5% SDS PAGE
GTX108495 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169