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IL2 antibody [HL2645]

Anti-IL2 antibody [HL2645] used in Western Blot (WB). GTX639104
Anti-IL2 antibody [HL2645] used in IHC (Paraffin sections) (IHC-P). GTX639104

Cat. No. GTX639104

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2645

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of Human IL2.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 2 , IL-2 , TCGF , lymphokine

Cellular Localization

Secreted

Background

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL2 antibody [HL2645] used in Western Blot (WB). GTX639104

GTX639104 WB Image

Untreated (–) and treated (+) Jurkat E6.1 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL2 antibody [HL2645] (GTX639104) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-IL2 antibody [HL2645] used in IHC (Paraffin sections) (IHC-P). GTX639104

GTX639104 IHC-P Image

IL2 antibody [HL2645] detects secreted IL2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IL2 stained by IL2 antibody [HL2645] (GTX639104) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations