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IL2 antibody

Orthogonal
Anti-IL2 antibody used in Flow cytometry (FACS). GTX101138
Anti-IL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101138
Anti-IL2 antibody used in Western Blot (WB). GTX101138

Cat. No. GTX101138

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164283

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 2 , IL-2 , TCGF , lymphokine

Cellular Localization

Secreted

Background

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL2 antibody used in Flow cytometry (FACS). GTX101138

GTX101138 FACS Image

IL2 antibody (GTX101138) detects IL2 by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: IL2 antibody (GTX101138) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-IL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101138

GTX101138 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using IL2(GTX101138) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IL2 antibody used in Western Blot (WB). GTX101138

GTX101138 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat E6.1 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL2 antibody (GTX101138) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169