APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 2 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
K-562 Cell Slide(GTX25535) , THP-1 Cell Lysate(GTX27913) , HL60 Cell Lysate(GTX27914)
Product Note
At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
IL-21 antibody was raised against a synthetic peptide corresponding to 15 amino acids near the center of human IL-21 precursor.The immunogen is located within the last 50 amino acids of IL-21.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 21 , CVID11 , IL-21 , Za11
Cellular Localization
Secreted
Background
A novel cytokine was recently identified in human and mouse and designated IL-21 , which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family (1,2). IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling (3,4). IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Database
Research Area
DATA IMAGES
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GTX85088 WB Image
WB analysis of HL-60 cell lysate in (A) the absence or (B) the presence of blocking peptide using GTX85088 IL21 antibody.
Working concentration : 1 μg/ml
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GTX85088 ICC/IF Image
ICC/IF analysis of K562 cells using GTX85088 IL21 antibody.
Working concentration : 2 μg/ml
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REFERENCE
There are currently no references for IL21 antibody (GTX85088). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL21 antibody (GTX85088). Be the first to share your experience with this product.
