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IL22 antibody

Anti-IL22 antibody used in IHC (Paraffin sections) (IHC-P). GTX109659
Anti-IL22 antibody used in Western Blot (WB). GTX109659
Anti-IL22 antibody used in ELISA (ELISA). GTX109659

Cat. No. GTX109659

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IL22 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human IL22. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 22 , IL-21 , IL-22 , IL-D110 , IL-TIF , ILTIF , TIFIL-23 , TIFa , zcyto18

Cellular Localization

Secreted

Background

Cytokine that contributes to the inflammatory response in vivo.

Database

Research Area

DATA IMAGES

Anti-IL22 antibody used in IHC (Paraffin sections) (IHC-P). GTX109659

GTX109659 IHC-P Image

IL22 antibody detects IL22 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
IL22 antibody (GTX109659) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IL22 antibody used in Western Blot (WB). GTX109659

GTX109659 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
12% SDS PAGE
GTX109659 diluted at 1:1000

Anti-IL22 antibody used in ELISA (ELISA). GTX109659

GTX109659 ELISA Image

An ELISA plate is coated with 50 μL of a IL-22 recombinant protein at 5 μg/mL. The coated protein is detected with anti-IL-22 antibody (GTX109659) at concentration ranged from 3.9 to 250 ng/mL.

REFERENCE

Application Reference

Wu Y et al. Exp Ther Med 2020; 20 (2) : 1012-1020 Interleukin-22 is elevated in the atrium and plasma of patients with atrial fibrillation and increases collagen synthesis in transforming growth factor-β1-treated cardiac fibroblasts via the JNK pathway.
Application : WB
Reactivity : Human
Ji YW et al. Mucosal Immunol 2017; (Epub) Lacrimal gland-derived IL-22 regulates IL-17-mediated ocular mucosal inflammation.
Application : IHC-P
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169