GeneTex
United States (US)

IL24 antibody [C2C3], C-term

GTX108472_39855_20151012_IHC-P_19011507_863.jpg
GTX108472_WB_19011507_729.jpg
GTX108472_39855_20151030_IHC-P_19011507_708.jpg

Cat No. GTX108472

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

A431
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Interleukin-24. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 24,C49A,Fisp,Il10B,Mda7,Mob5,St16,Il24

Cellular Localization

Secreted

Background

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108472_39855_20151012_IHC-P_19011507_863.jpg

GTX108472 IHC-P Image

Interleukin-24 antibody [C2C3], C-term detects Interleukin-24 protein at cytoplasm in human esophageal cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal cancer.
Interleukin-24 antibody [C2C3], C-term (GTX108472) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108472_WB_19011507_729.jpg

GTX108472 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX108472 diluted at 1:1000

GTX108472_39855_20151030_IHC-P_19011507_708.jpg

GTX108472 IHC-P Image

Interleukin-24 antibody [C2C3], C-term detects Interleukin-24 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Interleukin-24 antibody [C2C3], C-term (GTX108472) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169