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IL27 antibody (Biotin)

Anti-IL27 antibody (Biotin) used in Western Blot (WB). GTX48684

Cat No. GTX48684

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, IHC

Reactivity

Mouse
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000 - 1:10000
ELISA 1:20000 - 1:100000
IHC 1:1000 - 1:5000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide and 10 mg/mL Bovine Serum Albumin (BSA)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.

Purification

IgG fraction

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 27 , IL-27 , IL-27-A , IL-27p28 , IL27-A , Il30 , p28

Cellular Localization

Secreted

Background

The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer. The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.

Database

Research Area

DATA IMAGES

Anti-IL27 antibody (Biotin) used in Western Blot (WB). GTX48684

GTX48684 WB Image

Western Blot of Biotin Conjugated Rabbit anti-IL-27/p28 antibody. Load: 50 ng Mouse IL-27/p28 per lane. Blocking for 30 min at RT. Predicted/Observed size: 24 kDa for Mouse IL-27/p28. Other band(s): None.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 289