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IL27 antibody [MT27 + MT361]

Anti-IL27 antibody [MT27 + MT361] used in ELISA (ELISA). GTX02984

Cat. No. GTX02984

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MT27 + MT361

Isotype

 IgG1, IgG2b

Application

 ELISA, Sandwich ELISA

Reactivity

 Human
Package
250 μg ($399)
See all IL27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX02984 / GTX02920, Detection: GTX02919-02.
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Product Note

This antibody is able to detect native and recombinant human IL-27. Clone [MT27] binds to IL-27, and clone [MT361] binds to p28, which is part of the IL-27 heterodimer.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-27

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IL27,IL27A,IL27p28,IL30,interleukin 27,p28

Cellular Localization

Secreted

Background

The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL27 antibody [MT27 + MT361] used in ELISA (ELISA). GTX02984

GTX02984 ELISA Image

Sandwich ELISA analysis of human IL-27 protein using GTX02984 IL27 antibody [MT27 + MT361] as coating antibody and GTX02919-02 EBI3 antibody [MT140] (Biotin) as detecting antibody.
Substrate : pNPP

REFERENCE

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REVIEW

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Package List Price ($)
$ 399