WB analysis of recombinant mouse IL27 protein using GTX48686 IL27 antibody.
Loading : 0.25 μg
Dilution : 1:1000

Detection of recombinant IL27/p28 protein by anti-Mouse IL-27/p28 antibody (GTX48686). Recombinant mouse IL27/p28 was loaded on to an SDS-PAGE gel at 0.25 μg and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4ºC. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.