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IL29 antibody

Anti-IL29 antibody used in Western Blot (WB). GTX48682

Cat No. GTX48682

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ELISA 1:10,000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human IL-29 protein produced in E.coli.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interferon lambda 1 , IL-29 , IL29

Cellular Localization

Secreted

Background

IL-29 (Interleukin-29) is a secreted cytokine with immunomodulatory activity. It may play a role in antiviral immunity. It up-regulates MHC class I antigen expression. IL-29 is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Its induction is by viral infections or double stranded RNA.

Database

Research Area

DATA IMAGES

Anti-IL29 antibody used in Western Blot (WB). GTX48682

GTX48682 WB Image

Anti-human IL-29 antibody in western blot shows detection of recombinant human IL-29 raised in E.coli. Recombinant protein (0.1 μg, 19.9 kDa) was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with GeneTex's Anti-Human IL-29 antibody. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.

REFERENCE

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REVIEW

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Package List Price ($)
$ 289